乳业科学与技术2026，Vol.49Issue(2)：30-36,7.DOI:10.7506/rykxyjs1671-5187-20251104-078
基于分散液-液微萃取技术的驼乳中外源牛胰岛素快速检测
Rapid Detection of Exogenous Bovine Insulin in Camel Milk Based on Dispersive Liquid-Liquid Microextraction
摘要
Abstract
To addresses technical bottlenecks such as the complexity of the camel milk matrix and the interference of endogenous proteins with target detection,this study established an analytical method to detect exogenous bovine insulin in camel milk using dispersive liquid-liquid microextraction(DLLME)coupled with high performance liquid chromatography(HPLC).The extraction conditions and the instrumental parameters were optimized.The results showed that efficient enrichment and accurate quantification of target analytes in complex milk matrices were achieved by HPLC combined with DLLME using 0.01 mol/L HCl as the extractant,volume fraction 60%aqueous acetone as the extraction solvent,and n-hexane as the separation phase.The method exhibited good linearity for bovine insulin over the concentration ranges of 5-500 μg/mL(R2=0.999).Recoveries for blank sample of Bactrian camel milk from Dabancheng District,Ürümqi ranged from 64.1%to 86.9%at spiked levels of 5,50 and 500 μg/mL,with relative standard deviations(RSDs)of 4.9%-12.4%.The limit of detection and limit of quantification were 0.5 and 5 μg/mL,respectively.The DLLME-HPLC method is characterized by simple operation,short pretreatment time,and high analytical efficiency,providing technical support for rapid screening of exogenous bovine insulin in camel milk.关键词
驼乳/分散液-液微萃取/牛胰岛素/高效液相色谱Key words
camel milk/dispersive liquid-liquid microextraction/bovine insulin/high performance liquid chromatography分类
轻工纺织引用本文复制引用
杨新月,曹双瑜,赵艳坤,姜娜,木尼拉·地里夏提,武亚婷,马宪兰,陈贺..基于分散液-液微萃取技术的驼乳中外源牛胰岛素快速检测[J].乳业科学与技术,2026,49(2):30-36,7.基金项目
新疆农产品质量安全实验室开放基金资助课题项目(xjnkywdzc-2023003-pt2) （xjnkywdzc-2023003-pt2）
新疆维吾尔自治区奶产业技术体系资助项目(XJARS-11) （XJARS-11）
自治区重点研发计划项目(2025B04032-001) （2025B04032-001）
