乳业科学与技术2026，Vol.49Issue(2)：37-43,7.DOI:10.7506/rykxyjs1671-5187-20251104-073
食品中片球菌属鉴定方法比较分析
Comparative Analysis of Identification Methods for Pediococcus spp.in Food
摘要
Abstract
In this study,the traditional physiological and biochemical method,a VITEK automatic biochemical analyzer,the analytical profile index(API)biochemical identification system,real-time fluorescence quantitative polymerase chain reaction(real-time PCR)and matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS)were used comparatively to identify the species of Pediococcus spp.in food.The results showed that the traditional physiological and biochemical method was the most accurate but time-consuming and labor-intensive;the VITEK automatic biochemical analyzer gave unstable results as the database states that Pediococcus acidilactici is negative for amygdalin reaction,whereas some strains actually test positive;the API biochemical identification system yielded accurate results at 24 h of culture,while deviations occurred when the culture period was extended to 48 h;real-time PCR was time-saving,accurate,and suitable for popularization;the MALDI-TOF MS method had the shortest operation time but high equipment requirements,making it difficult to popularize in primary-level institutions.In summary,the traditional physiological and biochemical method,real-time PCR,and MALDI-TOF MS can all serve as standard methods for the identification of Pediococcus spp.in food.The results of this study can provide a technical basis for the formulation of relevant national standards.关键词
片球菌属/传统生理生化法/VITEK生化鉴定法/分析谱型指数生化鉴定系统/实时荧光定量聚合酶链式反应法/基质辅助激光解吸电离飞行时间质谱分类
轻工纺织引用本文复制引用
明若阳,周巍,刘雨蒙,王慧,李永艳,谢梦颖,张雅伦..食品中片球菌属鉴定方法比较分析[J].乳业科学与技术,2026,49(2):37-43,7.基金项目
河北省市场监管局科技计划项目(2024ZD03) （2024ZD03）
食品安全国家标准制定项目(spaq-2023-32) （spaq-2023-32）
