中国酿造2026，Vol.45Issue(4)：46-53,8.DOI:10.11882/j.issn.0254-5071.2026.04.007
基于代谢组学和转录组学探究不同通风比对地衣芽孢杆菌发酵产γ-聚谷氨酸的影响
Effects of Different Ventilation Ratios on γ-Polyglutamic Acid Production by Bacillus licheniformis Fermentation Based on Metabolomics and Transcriptomics
摘要
Abstract
To investigate the effects of different ventilation ratios on γ-polyglutamic acid(PGA)production by microbial fermentation,using Bacillus licheniformis BYBC1.21056 as the starting strain,the effects of different ventilation ratios(1.0:0.7,1.0:1.0,1.0:1.3,1.0:1.6,1.0:1.9)on intracellular metabolism and gene expression of strain BYBC1.21056 were analyzed by combining metabolomics and transcriptomics.The results showed that the high ventilation ratio(1.0:1.9)most favored γ-PGA synthesis,achieving a yield of 105.54 g/L,demonstrating that ventilation was a key factor influencing fermentation.Metabolomic analysis results showed that a total of 916,1 164,and 1 081 significantly different metabolites were identified between the experimental group and the control group,respectively,after fermentation 24,48 and 72 h.Among them,the unique significantly different metabolites were 293,563 and 412,respectively.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis results indicated that,compared with the control group,the significantly upregulated metabolic pathways for significantly different metabolites were tryptophan metabolism,nucleotide metabolism,glycerophospholipid metabolism,purine metabolism,nicotinic acid and nicotinamide metabolism,etc.in the experimental group after fermentation 24,48,and 72 h.Transcriptome analysis results revealed that there were 882 upregulated genes and 1 025 downregulated genes at 24 h,373 upregulated genes and 506 downregulated genes at 48 h,and 461 upregulated genes and 394 downregulated genes at 72 h.The results showed that increasing the ventilation ratio altered gene expression in B.licheniformis,thereby regulating its metabolism and affecting γ-PGA synthesis.关键词
γ-聚谷氨酸/地衣芽孢杆菌/通风比/代谢组学/转录组学Key words
γ-polyglutamic acid/Bacillus licheniformis/ventilation ratio/metabolomics/transcriptomics分类
化学化工引用本文复制引用
贾巧帅,王彬旭,王慧超,乔长晟,赵廷彬,郑国保..基于代谢组学和转录组学探究不同通风比对地衣芽孢杆菌发酵产γ-聚谷氨酸的影响[J].中国酿造,2026,45(4):46-53,8.基金项目
宁夏回族自治区重点研发计划项目(2022BEG02006) （2022BEG02006）
吉林省科技发展计划项目(20250204039YY) （20250204039YY）
