包装与食品机械2026，Vol.44Issue(2)：1-13,13.DOI:10.3969/j.issn.1005-1295.2026.02.001
基于GOD@Fe3O4@SiO2-NH2-CDI NPs构建葡萄糖生物传感器
Construction of glucose biosensors based on GOD@Fe3O4@SiO2-NH2-CDI NPs
摘要
Abstract
To address the issues of poor stability,high equipment cost,and complex operational procedures in current glucose concentration detection methods,a surface functionalization modification approach was adopted.The effects of acetic acid,3-aminopropyltriethoxysilane(APTES),and N,N'-carbonyldiimidazole(CDI)on the modification of magnetic nanoparticles were investigated using conductivity measurements,Fourier transform infrared spectroscopy(FTIR)and Bradford enzyme loading assays.A novel enzyme carrier material,Fe3O4@SiO2-NH2-CDI NPs,was successfully prepared.This material was then integrated with an SBA-40E sensor to construct a novel biosensor for glucose concentration detection.Electrochemical detection was performed,and the performance was compared in parallel with conventional biosensors for validation.The results showed that the Fe3O4@SiO2-NH2-CDI NPs exhibited an enzyme loading capacity of 116 mg/g,which was 7.73 times higher than that of Fe3O4@SiO2 NPs.The GOD@Fe3O4@SiO2-NH2-CDI NPs biosensor demonstrated a linear response range for glucose detection of 0.25-1.25 mM(R2≥0.994),maintained excellent linearity after 25 d of storage,with a detection limit of 0.054 mM and a sensitivity of 58.9 μA·L/(mol·cm2).The relative standard deviations(RSD)for standard sample detection over 15 d were all less than or equal to 5.0%.For glucose beverage detection,the RSD ranged from 0.90%to 2.30%,outperforming conventional GOD biosensors(which showed RSD of 2.20%to 3.20%)in terms of detection stability and accuracy.The service life of the developed biosensor was 1.79 to 3.13 times longer than that of other sensors(8-14 d),and the detection limit was reduced by 49.3%to 80.0%compared to most similar sensors.This research provides a reference for the development of biosensors towards greater efficiency,accuracy and convenience.关键词
功能化修饰/磁性纳米材料/电化学生物传感器/固定化酶/葡萄糖检测Key words
functionalized modification/magnetic nanomaterials/electrochemical biosensor/immobilized enzyme/glucose detection分类
轻工纺织引用本文复制引用
王志华,李国琳,苗田田,马耀宏,刘庆艾..基于GOD@Fe3O4@SiO2-NH2-CDI NPs构建葡萄糖生物传感器[J].包装与食品机械,2026,44(2):1-13,13.基金项目
国家重点研发计划项目(2022YFC3401302) （2022YFC3401302）
济南市深化市校融合发展促进科技成果转化的若干措施(2025版)项目(202228084) （2025版）
