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超声辅助低共熔溶剂提取槐米多糖的工艺优化

王乾芬 王超琪 姜明均 贺珧珈 徐小博 张艳芳

包装与食品机械2026,Vol.44Issue(2):43-49,7.
包装与食品机械2026,Vol.44Issue(2):43-49,7.DOI:10.3969/j.issn.1005-1295.2026.02.005

超声辅助低共熔溶剂提取槐米多糖的工艺优化

Process optimization of ultrasound-assisted deep eutectic solvent extraction of Flos sophorae immaturus polysaccharides

王乾芬 1王超琪 1姜明均 1贺珧珈 1徐小博 1张艳芳1

作者信息

  • 1. 新乡学院 生物工程学院,河南 新乡 453003
  • 折叠

摘要

Abstract

To efficiently extract and utilize Flos sophorae immaturus polysaccharides(FSIP),an ultrasound-assisted deep eutectic solvent(DES)extraction method was employed.Using the polysaccharide yield as the evaluation index,the extraction process was optimized through single-factor experiments and response surface methodology,and the antioxidant activity of the extracted polysaccharides was determined.The results showed that the optimal solvent system was a DES composed of choline chloride and ammonium acetate.The optimal extraction conditions were as follows:DES molar ratio of 1∶2,DES water mass fraction of 50%,solid-to-liquid ratio of 1∶38(g/mL),ultrasonic power of 240 W,ultrasonic temperature of 40℃,and ultrasonic time of 30 min.Under these conditions,the yield of FSIP reached 8.80%.The IC50 values for DPPH and ABTS radical scavenging activities were 0.422 mg/mL and 0.240 mg/mL,respectively.At a polysaccharide concentration of 0.25 mg/mL,the reducing powers for iron ions and copper ions were 8.26 μmol/mL and 30.32 μmol/mL,respectively,indicating that FSIP possesses some antioxidant activity.This research provides a foundation for the efficient extraction and high-value utilization of FSIP.

关键词

槐米/多糖/低共熔溶剂/工艺优化/抗氧化

Key words

Flos sophorae immaturus/polysaccharides/deep eutectic solvent/process optimization/antioxidant activity

分类

轻工纺织

引用本文复制引用

王乾芬,王超琪,姜明均,贺珧珈,徐小博,张艳芳..超声辅助低共熔溶剂提取槐米多糖的工艺优化[J].包装与食品机械,2026,44(2):43-49,7.

基金项目

河南省科技攻关项目(252102110088) (252102110088)

河南省生物医药产业联合基金项目(235101610045) (235101610045)

河南省高等教育教学改革研究与实践项目(2023SJGLX094Y) (2023SJGLX094Y)

包装与食品机械

1005-1295

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