现代食品科技2026，Vol.42Issue(4)：22-31,10.DOI:10.13982/j.mfst.1673-9078.2026.4.0049
辣木叶多糖通过TLR4/NF-κB信号通路减轻横纹肌溶解致急性肾损伤
Moringa oleifera Leaf Polysaccharide Alleviates Acute Renal Injury Induced by Rhabdomyolysis via TLR4/NF-κB Signaling Pathway
摘要
Abstract
The aim of this study was to investigate the protective effects of Moringa oleifera leaf polysaccharide(MLP)on rhabdomyolysis-induced acute kidney injury(RM-AKI)and the potential mechanisms of such effects.A mouse RM-AKI model and a ferrous myoglobin-induced injury model were established in renal tubular epithelial cells(HK-2).Biochemical kits were used to measure the levels of creatinine(Cr),blood urea nitrogen(BUN),and creatine kinase(CK)in mouse serum,as well as cytokines(IL-1β,IL-6,and TNF-α)in kidney tissue.Furthermore,hematoxylin and eosin staining and TUNEL staining were used to evaluate pathological changes and apoptosis in kidney tissue.Western blotting was performed to evaluate the expression of key proteins in both kidney tissue and HK-2 cells.The results revealed that,compared with the model group,the levels of Cr,BUN,CK,IL-1β,IL-6,and TNF-α in the high-dose MLP-treated mice decreased by 67.87％,43.03％,21.39％,31.05％,54.20％,and 51.66％,respectively(P＜0.001).In addition,HK-2 apoptosis was significantly inhibited,and oxidative stress levels notably decreased in ferrous myoglobin-induced HK-2 cells.A significant downregulation in the expression of TLR4,P-p65/p65,and cleaved caspase-3,both in vivo and in vitro,was observed after MLP treatment.These results indicate that MLP has a significant protective effect on RM-AKI,which provide a scientific basis for the clinical treatment of patients with RM-AKI.关键词
辣木叶多糖/横纹肌溶解/急性肾损伤/TLR4/NF-κB信号通路/氧化应激Key words
Moringa oleifera leaf polysaccharide/rhabdomyolysis/acute kidney injury/TLR4/NF-κB signaling pathway/oxidative stress引用本文复制引用
黄开兰,张明,卢娟,田洋,白忠彬,马啸..辣木叶多糖通过TLR4/NF-κB信号通路减轻横纹肌溶解致急性肾损伤[J].现代食品科技,2026,42(4):22-31,10.基金项目
云南省科技计划项目-农业联合专项(202301BD070001-195) （202301BD070001-195）
云南省兴滇英才青年人才专项(YNWR-QNBJ2018-173) （YNWR-QNBJ2018-173）
