现代食品科技2026，Vol.42Issue(4)：85-93,9.DOI:10.13982/j.mfst.1673-9078.2026.4.0407
fliE基因对单增李斯特菌持留能力形成的作用分析
Role of the fliE Gene in the Formation of Persistence in Listeria monocytogenes
摘要
Abstract
To analyze the impact of the fliE gene on the persistence of Listeria monocytogenes,a fliE deletion mutant was successfully constructed using homologous recombination technology.Growth capacity assays demonstrated that the ΔfliE strain reached the stationary phase at an OD600 of 0.64,whereas the parental strain reached the stationary phase at an OD600 of 0.66.No significant difference in growth capability was observed between the parental and ΔfliE strains(P＞0.05).Motility assays demonstrated that the migration diameters of the parental and ΔfliE strains in a semi-solid agar medium were 6.00±0.05 mm and 6.03±0.05 mm,respectively,suggesting no significant difference in motility(P＞0.05).The biofilm formation capability of the ΔfliE strain was assessed using the crystal violet staining method.Compared with the parental strain,the ability of the ΔfliE strain to form mixed biofilms with Weissella confusa W4-1 and WL5-1 decreased to 71.55％and 74.77％(P＜0.01),respectively.Disinfectant tolerance assays and analysis based on confocal laser scanning microscopy(CLSM)suggested that the survival ability of L.monocytogenes in mixed biofilms formed by co-culturing the ΔfliE strain with Weissella confusa was significantly reduced.These results demonstrated that deletion of the fliE gene not only affected the biofilm formation ability of L.monocytogenes but also reduced its tolerance to quaternary ammonium disinfectants.The findings of this study provide a theoretical foundation for further understanding the molecular mechanisms underlying the persistence of hypervirulent,persistent-genotype L.monocytogenes strains.关键词
单增李斯特菌/鞭毛/fliE基因/生物被膜/季铵盐类消毒剂Key words
Listeria monocytogenes/flagellum/fliE gene/biofilm/quaternary ammonium compounds引用本文复制引用
冯瑞珍,叶志伟,蔡佳怡,程健恒,张颖,陈谋通..fliE基因对单增李斯特菌持留能力形成的作用分析[J].现代食品科技,2026,42(4):85-93,9.基金项目
国家自然科学基金项目(32222068) （32222068）
广东省基础与应用基础研究基金项目(2022B1515020068) （2022B1515020068）
广东省科学院青年人才专项(2023GDASQNRC-0102) （2023GDASQNRC-0102）
