食品工业科技2026，Vol.47Issue(11)：65-76,12.DOI:10.13386/j.issn1002-0306.2025040217
基于体外化学、细胞和线虫模型评价小黄姜提取物及其复配物的协同降血糖活性
Evaluation of Synergistic Hypoglycemic Activity of Small Yellow Ginger Extract and Its Complexes Based on in Vitro Chemical,Cellular,and Nematode Models
摘要
Abstract
To develop natural product complexes with synergistic hypoglycemic effects,small yellow ginger extract(GE)was used as the main material with dried tangerine peel(Citrus reticulata Blanco)and hawthorn(Crataegus pinnatifida Bge.)extracts(DTPE and CPE)as auxiliary materials.The α-glucosidase activity inhibition assay was integrated with the isobologram method and Chou-Talalay combination index method to comprehensively analyze and evaluate the α-glucosi-dase inhibitory activities and combination indices of the individual and combined extracts of small yellow ginger,dried tan-gerine peel,and hawthorn.Subsequently,the complex combinations exhibiting synergistic hypoglycemic effects were fur-ther investigated for their hypoglycemic activities using an insulin-resistant HepG2(IR-HepG2)cell model and a Caenorhabditis elegans(C.elegans)hyperglycemic model.The results showed that GE-DTPE(1:2),GE-CPE(1:2)and GE-CPE(1:3)combinations exhibited synergistic hypoglycaemic effects(CI:0.759,0.837,and 0.861,respectively).In the IR-HepG2 cell experiments,when the sample concentration was 400 μg/mL,the GE,GE-DTPE(1:2),GE-CPE(1:2),GE-CPE(1:3),and acarbose increased IR-HepG2 cell glucose consumption by 16.86%,18.09%,22.51%,18.97%,and 40.57%,and boosted glycogen synthesis by 13.18%,14.90%,17.10%,15.67%,and 24.60%,respectively.In the high-glucose C.ele-gans model,GE-CPE(1:2)at a sample concentration of 200 μg/mL exhibited the strongest hypoglycemic potential com-pared to the high-glucose control group.It increased GSH and SOD levels by 69.89%and 46.99%,respectively,while reducing MDA,intracellular glucose,triglyceride,and lipid accumulation by 37.43%,66.10%,56.43%,and 47.35%,respectively.This study provides a theoretical reference for the study of synergistic hypoglycaemic activity of small yellow ginger extract series and the development of compound hypoglycaemic functional foods.关键词
小黄姜提取物/α-葡萄糖苷酶/等效线图解法/Chou-Talalay/联合指数/协同降血糖Key words
small yellow ginger extract/α-glucosidase/isobolographic method/Chou-Talalay/combination index/syner-gistic hypoglycaemia分类
轻工纺织引用本文复制引用
杨秋变,张笑莹,陈沛航,徐嘉欣,苗建银..基于体外化学、细胞和线虫模型评价小黄姜提取物及其复配物的协同降血糖活性[J].食品工业科技,2026,47(11):65-76,12.基金项目
广东省驻镇扶村科技特派员项目(E22047). （E22047）
