食品科学2026，Vol.47Issue(7)：54-60,7.DOI:10.7506/spkx1002-6630-20251021-146
罗非鱼皮胶原蛋白肽结构鉴定、潜在抗冻活性及机制分析
Structural Identification,Antifreeze Potential and Mechanism of Action of Peptides from Tilapia Skin Collagen
摘要
Abstract
Using a combination of molecular docking and in vitro activity evaluation,three antifreeze peptides(i.e.,PQGPVGNTGPKG,LSGPTGEAGRE and GGRGPEGPAGAR)were selected from 386 tilapia skin collagen peptides,and their interactions with ice crystals were explored.The thermal hysteresis activities of these peptides were 1.57,1.82,and 1.22℃,respectively.After freeze-thaw cycles,catalase retained 57.0%,66.7%,and 52.3%of its initial activity in the presence of the three antifreeze peptides,respectively,significantly higher than that of the blank control(32%).Molecular docking revealed that hydrogen bonding was the primary driving force for the stable binding of these antifreeze peptides to ice crystal planes.These findings offer an efficient strategy for converting tilapia processing by-products into high-value commercial antifreeze agents.关键词
胶原蛋白/抗冻肽/热滞活性/过氧化氢酶活性/分子对接Key words
collagen/antifreeze peptide/thermal hysteresis/catalase activity/molecular docking分类
轻工纺织引用本文复制引用
李诗诗,赵文竹,魏莱,杨维,于志鹏..罗非鱼皮胶原蛋白肽结构鉴定、潜在抗冻活性及机制分析[J].食品科学,2026,47(7):54-60,7.基金项目
海南省重点研发项目(ZDYF2024XDNY204) （ZDYF2024XDNY204）
