食品科学2026，Vol.47Issue(7)：101-108,8.DOI:10.7506/spkx1002-6630-20251018-096
应用噬菌体展示随机七肽库筛选花生Ara h 5蛋白的模拟表位
Screening for Mimotopes for the Peanut Allergen Ara h 5 Using a Phage Display Random Heptapeptide Library
摘要
Abstract
This study aimed to screen for mimotopes for the peanut allergen Ara h 5 using a phage display random heptapeptide library.Recombinant Ara h 5,obtained via a prokaryotic expression system,was used as an immunogen to generate antibodies in a mouse model.Polyclonal IgE antibodies against Ara h 5 were purified from the antiserum by affinity chromatography.Using these Ara h 5 IgE antibodies as the target,three rounds of biopanning were performed against the phage display heptapeptide library.Positive phage clones were isolated and sequenced.Three mimotope sequences,FHWWYLK,WETIYSR,and GPLWNVN,were identified.These mimotopes provide an important foundation for the development of blocking antibodies or epitope-specific immunotherapy for peanut allergy.关键词
花生过敏/Ara h 5/噬菌体展示技术/模拟表位Key words
peanut allergy/Ara h 5/phage display technology/mimotope分类
轻工纺织引用本文复制引用
王俊娟,秦培友,王丹,赵圆圆,彭郁,赵晓燕,车会莲..应用噬菌体展示随机七肽库筛选花生Ara h 5蛋白的模拟表位[J].食品科学,2026,47(7):101-108,8.基金项目
国家自然科学基金青年科学基金项目(32402246) （32402246）
北京市农林科学院青年科研基金项目(QNJJ202412) （QNJJ202412）
北京市农林科学院2025年度国际科技合作平台建设专项(GHPT2025-05) （GHPT2025-05）
