食品科学2026，Vol.47Issue(8)：32-42,11.DOI:10.7506/spkx1002-6630-20260101-001
不同分子结构甜菊糖苷与大豆蛋白的结合行为及对其感官特性的影响
Binding Behavior of Steviol Glycosides with Diverse Molecular Structures to Soy Proteins and Its Influence on Their Sensory Properties
摘要
Abstract
Three steviol glycosides(SGs)with distinct molecular structures—rebaudioside A(Reb A),stevioside(STV),and rebaudioside M(Reb M)—were selected to systematically investigate their binding behaviors with two major soy protein components,glycinin(11S)and β-conglycinin(7S),in aqueous solutions.Furthermore,the impact of these interactions on the sensory properties of SGs was explored.The results indicated that SGs formed complexes with proteins primarily through hydrogen bonding and hydrophobic interactions,with their binding affinity dually regulated by the molecular structure of SGs and the subunit structure of proteins.Specifically,Reb M,with the highest number of sugar moieties,exhibited the strongest binding capacity,followed by Reb A,with STV showing the weakest affinity.Notably,the binding ratios for all SG concentrations were below 20%.Regarding the two protein fractions,11S showed a higher binding capacity for SGs than did 7S.Sensory evaluation revealed that SG sweetness was regulated by soy proteins in solutions in a concentration-dependent manner.Binding with 0.1%soy proteins mitigated the negative sensory attributes of Reb A and STV at a low concentration(0.1%)without significantly compromising their sweetness intensity.At a medium SG concentration(0.3%),it slightly attenuated the sweetness intensity of the three SGs but still inhibited some negative attributes of Reb A and STV.However,at a high SG concentration(0.5%),the masking effect on the bitterness and astringency was limited.关键词
甜菊糖苷/大豆蛋白/分子结构/结合行为/感官特性Key words
steviol glycosides/soy proteins/molecular structure/binding behavior/sensory properties分类
轻工纺织引用本文复制引用
冉佳鑫,郑键欣,杨韵仪,郭健,万芝力,杨晓泉..不同分子结构甜菊糖苷与大豆蛋白的结合行为及对其感官特性的影响[J].食品科学,2026,47(8):32-42,11.基金项目
"十四五"国家重点研发计划重点专项(2022YFD2101302) （2022YFD2101302）
国家自然科学基金面上项目(32572748) （32572748）
广东省自然科学基金杰出青年基金项目(2024B1515020081) （2024B1515020081）
