食品科学2026，Vol.47Issue(8)：95-103,9.DOI:10.7506/spkx1002-6630-20251105-023
红阳猕猴桃多糖结构解析及对秀丽隐杆线虫抗衰老作用
Structural Analysis of Actinidia chinensis cv.Hongyang Polysaccharides and Their Anti-aging Effect on Caenorhabditis elegans
摘要
Abstract
To investigate the structure of polysaccharides from Actinidia chinensis cv.Hongyang and their antioxidant and anti-aging effects on Caenorhabditis elegans,crude polysaccharides from A.chinensis cv.Hongyang(ACP)were prepared by ultrasound-assisted extraction.ACP were purified sequentially on DEAE-52 cellulose and Sephadex G-100 columns,yielding a homogenous polysaccharide(ACP-3-G).The molecular structure of ACP-3-G was characterized using high performance liquid chromatography(HPLC),high performance gel permeation chromatography(HPGPC),and nuclear magnetic resonance(NMR)spectroscopy.Finally,the effects of different concentrations of ACP-3-G on the lifespan,motility,heat stress resistance,and oxidative stress resistance of C.elegans as a model organism were evaluated to its anti-aging potential.The results showed that the yield of ACP-3-G was(73.42±1.74)%,with higher contents of polysaccharide and uronic acid than ACP.In addition,ACP-3-G was a polysaccharide with a molecular mass of 120.6 kDa.Its monosaccharide composition included mannose(0.367%),rhamnose(2.384%),galacturonic acid(58.859%),glucose(4.501%),galactose(23.942%),and arabinose(9.307%).NMR analysis indicated that the main chain of the polysaccharide was primarily composed of disubstituted sugar residues →1)-α-Araf-(5→,→1)-α-GalpA-(4→,→1)-β-Galp-(4→,and trisubstituted sugar residues →1,3)-β-GalpA-(4→,→1,4)-β-Galp-(6→,and →1,2)-α-GalpA-(4→.Furthermore,ACP-3-G intervention at concentrations of 10,50,100,500,and 1 000 μg/mL significantly improved the lifespan,motility,heat stress resistance,and oxidative stress resistance of C.elegans compared with the control group,with the most pronounced effects observed at 1 000 μg/mL.In conclusion,ACP-3-G is a polysaccharide with antioxidant and anti-aging properties.关键词
红阳猕猴桃/多糖/结构解析/秀丽隐杆线虫/抗衰老/抗氧化Key words
Actinidia chinensis cv.Hongyang/polysaccharides/structural analysis/Caenorhabditis elegans/anti-aging/antioxidant分类
轻工纺织引用本文复制引用
吴应梅,徐敏,刘爽,陈嘉俊,张林,伍莲莲,刘小凡,王天玉,唐华丽..红阳猕猴桃多糖结构解析及对秀丽隐杆线虫抗衰老作用[J].食品科学,2026,47(8):95-103,9.基金项目
山东省科学技术厅鲁渝科技协作计划项目(2023LYXZ005) （2023LYXZ005）
重庆市基础研究与前沿探索项目(cstc2018jcyjAX0770) （cstc2018jcyjAX0770）
重庆市自然科学基金面上项目(cstb2022nscq-MSX1661) （cstb2022nscq-MSX1661）
