食品科学2026，Vol.47Issue(8)：104-112,9.DOI:10.7506/spkx1002-6630-20251022-160
复合磷酸盐修饰提升乳清蛋白热稳定性与表面特性的机制
Mechanism by Which Modification with Phosphate Mixtures Improve the Thermal Stability and Surface Properties of Whey Protein Isolate
摘要
Abstract
This study investigated the hydrothermal phosphorylation modification of whey protein isolate(WPI90)using mixtures of sodium hexametaphosphate(SHMP)and tetrasodium pyrophosphate decahydrate(SPP)at different mass ratios(1:1,1:2,and 2:1)applied at varying mass concentrations(0.03,0.06,and 0.09 g/100 mL).The secondary structure,thermal stability,solubility,foaming properties,emulsifying properties,and water-holding capacity of WPI were determined before and after phosphorylation.The results indicated that the negative charges introduced by phosphorylation altered the structural characteristics of WPI.This modification resulted in an increase in α-helix content in the secondary structure and induced noticeable microstructural changes.Phosphate addition enhanced the thermal stability and kinetic stability after heating to varying degrees;the phosphorylated WPI solutions remained clear and transparent after heating,showing a turbidity approximately 80%lower than that of unmodified WPI90.All phosphorylation modifications altered the foaming and emulsifying properties of WPI.Notably,the addition of the 2:1 mixture at 0.06 and 0.09 g/100 mL resulted in a 3-to 4-fold increase in the emulsion stability of WPI and increased the water-holding capacity from 20%to 50%and 90%,respectively,demonstrating a remarkable modification effect.This study provides a feasible approach to address the stability limitations of WPI in food processing.关键词
复合磷酸盐/湿热磷酸化/乳清蛋白/功能特性Key words
phosphate mixtures/hydrothermal phosphorylation/whey protein isolate/functional properties分类
轻工纺织引用本文复制引用
董暄,路茹青,逄晓阳,王筠钠,吕加平,李红娟,张书文..复合磷酸盐修饰提升乳清蛋白热稳定性与表面特性的机制[J].食品科学,2026,47(8):104-112,9.基金项目
中国乳制品工业协会乳业科技创新基金——全优专项 （）
云南省重大专项(202402AE090033) （202402AE090033）
克拉玛依市重点研发计划项目(2024zdyf0007) （2024zdyf0007）
国家奶牛产业技术体系项目(CARS-36) （CARS-36）
