食品与发酵工业2026，Vol.52Issue(9)：205-212,8.DOI:10.13995/j.cnki.11-1802/ts.044009
筛选用于分离纯化芝麻抗氧化肽的树脂
Screening of the suitable resin for separation and purification of sesame antioxidant peptides
摘要
Abstract
To identify the appropriate resin for the separation and purification of the sesame antioxidant peptides,the performance of 20 resins belonging to 6 types on the static adsorption of sesame protein hydrolysate were investigated with peptide recovery,impurity resi-due and antioxidant activity as evaluation indexes,the molecular weight distribution and composition of absorbed and unabsorbed peptides originated from macroporous resin D3520 were analyzed,finally,the difference on structural characteristics between absorbed and unab-sorbed peptides was explored.Results showed that the type and structure of resin significantly affected the behavior of isolation and purifi-cation of sesame antioxidant peptides by resins,such as ion exchange resins were suitable for removing impurities(amino acid,protein,salt,pigment)in enzymatic hydrolysate,while the nonpolar macroporous resin was good at enriching the sesame antioxidant peptide.Ac-cording to the performance on antioxidant activity and recovery of peptide,impurity removal,D3520 was suitable to separate and purify the sesame antioxidant peptide.Compared with the unabsorbed peptides after treated by D3520,the molecular weight distribution of absorbed peptides was concentrated to the low molecular weight,the kinds of absorbed peptides were1.6 times more than unabsorbed peptides,the absorbed peptides showed higher hydrophobicity,lower steric hindrance,and higher pI.These findings provide the preliminary basis for optimizing the separation and purification of sesame antioxidant peptide or other peptides by resin,and also provide the date reference for exploring the adsorption mechanism of resin to peptides.关键词
大孔树脂/离子交换树脂/抗氧化活性/杂质脱除/多肽组成Key words
macroporous resin/ion exchange resin/antioxidant activity/impurity removal/peptide composition引用本文复制引用
芦鑫,张丽霞,孙晓静,孙强,宋国辉,黄纪念..筛选用于分离纯化芝麻抗氧化肽的树脂[J].食品与发酵工业,2026,52(9):205-212,8.基金项目
河南科技研发计划联合基金(应用攻关)项目(242103810085) （应用攻关）
国家特油产业技术体系(CARS-14) （CARS-14）
