中外葡萄与葡萄酒Issue(3)：30-43,14.DOI:10.13414/j.cnki.zwpp.2026.03.004
欧亚种酿酒葡萄硬枝扦插生根过程插条基部代谢物质变化研究
Changes of Metabolic Compounds in Vitis vinifera L.during Rooting Process of Hardwood Cuttings
摘要
Abstract
To elucidate the metabolic changes in the basal region of cuttings during the rooting process of wine grape cuttings and their relationship with cutting genotype,this study employed cuttings from three Vitis vinifera L.varieties('Cabernet Sauvignon''Petit Manseng',and'Petit Verdot')to analyse changes in carbon sources(soluble sugars and starch),nitrogen sources(amino acids and water-soluble proteins),phenolic compounds(phenolic acids,flavonols,free flavan-3-ols,and stilbenoids),and plant hormones(IAA,ABA,JA,etc.)at the base of cuttings during rooting.Correlation analysis was conducted between these changes and callus formation rates and rooting rates.Results indicated significant differences in metabolic dynamics among the three cultivars.'Cabernet Sauvignon'exhibited a high IAA/ABA ratio during adventitious root formation,accompanied by starch hydrolysis and sustained accumulation of amino acids and water-soluble proteins,yielding the highest rooting rate.'Petit Manseng'exhibited slow abscisic acid degradation and delayed carbon mobilisation,resulting in delayed rooting;'Petit Verdot'showed low soluble sugar concentrations and decreased water-soluble protein levels during the later stages of cuttings,with persistently high levels of phenolic acids and free flavan-3-ols,leading to low rooting rates.Correlation analysis indicates that soluble sugar,starch,amino acids and water-soluble proteins,quercetin glycosides,epicatechin and resveratrol among phenolic compounds,and most plant hormones are positively correlated with rooting.Conversely,high concentrations of ABA,JA-Ile,SA and other phenolic compounds are negatively correlated with rooting.The rooting process of grapevine hardwood cuttings is a complex physiological process synergistically regulated by hormone signalling,carbon-nitrogen metabolism,and phenolic compounds.关键词
欧亚种酿酒葡萄/硬枝扦插/初级代谢物/植物激素/酚类物质Key words
Vitis vinifera L./hardwood cuttings/primary metabolites/phytohormone/phenolic compounds分类
农业科技引用本文复制引用
魏嘉彦,李明玉,田萌勃,沈甜,徐美隆,何非,于可济,王军..欧亚种酿酒葡萄硬枝扦插生根过程插条基部代谢物质变化研究[J].中外葡萄与葡萄酒,2026,(3):30-43,14.基金项目
财政部和农业农村部:国家葡萄产业技术体系项目(CARS-29)宁夏回族自治区重点研发计划重大项目(2024BBF01002-03) （CARS-29）
