中外葡萄与葡萄酒Issue(3)：78-86,9.DOI:10.13414/j.cnki.zwpp.2026.03.009
葡萄黑曲霉病病原菌的分离鉴定及防治药剂筛选
Isolation and Identification of the Pathogen Causing Grape Black Mold Rot and Screening of Fungicides for Its Control
摘要
Abstract
To identify the pathogen causing black mold disease in'Shine Muscat'grapes,this study,a combination of field disease investigation,conducted systematic identification through tissue isolation,Koch's postulates validation,morphological observation,and molecular identification.The toxicity of fungicides was determined in vitro using the mycelial growth rate method,and the control efficacy was evaluated through in vitro fruit inoculation assays.Field surveys showed that seedling type significantly influenced the incidence of grape black mold rot.Both morphological and molecular analyses identified the causal pathogen as Aspergillus tubingensis,and wounds were determined to be the key infection route.The effect of greenhouse type on disease severity was not significant.The results of laboratory fungicide toxicity tests showed significant differences in the inhibitory effects of the six tested fungicides against A.tubingensis.Among them,azoxystrobin and difenoconazole exhibited the strongest antifungal activity,with EC50 values of 120.962 mg·L-1 and 167.016 mg·L-1,and in vitro control efficacies of 48.97%and 46.29%,respectively.Flusilazole and carbendazim showed moderate inhibition,with EC50 values of 301.717 mg·L-1 and 359.174 mg·L-1,and in vitro control efficacies of 39.83%and 43.43%,respectively.In contrast,tebuconazole and pyraclostrobin exhibited no significant inhibitory effects.This study identified A.tubingensis as the causal agent of black mold disease on'Shine Muscat'grapes in Shandong production region.Azoxystrobin and difenoconazole can serve as candidate fungicides for the control of black mold disease in grape production.关键词
葡萄/塔宾曲霉/病原鉴定/药剂筛选Key words
grape/Aspergillus tubingensis/pathogen identification/fungicide screening分类
农业科技引用本文复制引用
任月华,姚晓伟,尹向田,李廷刚,魏彦锋,李楠洋,袁丽芳..葡萄黑曲霉病病原菌的分离鉴定及防治药剂筛选[J].中外葡萄与葡萄酒,2026,(3):78-86,9.基金项目
国家自然科学基金青年项目(32402337) （32402337）
山东省农科院创新工程(CXGC2025C15) （CXGC2025C15）
