食品科学2026，Vol.47Issue(9)：44-51,8.DOI:10.7506/spkx1002-6630-20250915-108
基于代谢组学探究日粮添加丙酸钠对羔羊肌肉代谢和肉品质的影响
Metabolomic Elucidation of the Effect of Dietary Sodium Propionate Addition on Lamb Muscle Metabolism and Meat Quality
摘要
Abstract
To investigate the effect of dietary sodium propionate(SP)on muscle metabolism and meat quality in lambs,12 three-month-old Dorper(ewe)×Suffolk(ram)crossbred lambs with good body condition and a body mass of(22.70±1.91)kg were selected and randomly divided into a control group(fed a basal diet)and an SP group(fed 20 g of SP per animal per day+a basal diet)for a 90-day feeding trial.After slaughter,the longissimus dorsi muscle was collected for the determination of fatty acid composition and muscle metabolic profiles using widely targeted metabolomics based on gas chromatography-mass spectrometry(GC-MS)and ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The results showed that dietary SP significantly increased the protein content in the longissimus dorsi muscle(P<0.05),and decreased the redness,yellowness,pH45 min and pH24 h values(P<0.05).Meanwhile,dietary SP significantly increased the contents of nonadecanoic acid,arachidic acid,heneicosanoic acid,cis-11-eicosenoic acid,cis-13,16-docosadienoic acid,cis-8,11,14-eicosatrienoic acid,and α-linolenic acid in the muscle(P<0.05),and decreased the content of cis-10-pentadecenoic acid(P<0.05).Widely targeted metabolomics analysis identified 1 032 metabolites in the longissimus dorsi muscles of the two groups,among which 152 substances were significantly differential metabolites(SDMs).In the SP group,3-iodo-L-tyrosine,(R)-2-hydroxy-3-phenylpropionic acid,lysophosphatidylserine(18:0),5'-adenylyl sulfate,and DL-glyceraldehyde-3-phosphate were significantly upregulated.However,carnitine C9:1,carnitine C18:1,carnitine C13:0,and carnitine C9:0 were significantly downregulated.Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that these SDMs were significantly enriched in metabolic pathways such as protein digestion and absorption,fatty acid elongation,amino acid biosynthesis,and unsaturated fatty acid biosynthesis.In conclusion,dietary SP may affect the metabolic homeostasis of lamb muscle by regulating amino acid and lipid metabolism via multiple signaling pathways and targets,thereby increasing muscle protein content,optimizing muscle fatty acid composition,and ultimately improving the eating quality and nutritional value of lamb meat.关键词
丙酸钠/羔羊/肌肉代谢/肉品质Key words
sodium propionate/lambs/muscle metabolism/meat quality分类
轻工纺织引用本文复制引用
曲洪波,张月,刘俊廷,郭月英,孙立娜,靳烨..基于代谢组学探究日粮添加丙酸钠对羔羊肌肉代谢和肉品质的影响[J].食品科学,2026,47(9):44-51,8.基金项目
国家自然科学基金地区科学基金项目(32160589) （32160589）
中央引导地方科技发展资金项目(2024ZY0020) （2024ZY0020）
内蒙古自然科学基金项目(2025MS03004 （）
2022MS03055) （）
