食品科学2026，Vol.47Issue(9)：190-202,13.DOI:10.7506/spkx1002-6630-20251113-101
知母多糖的制备、表征及其对衰老小鼠脑组织和肠道菌群的影响
Preparation and Characterization of Anemarrhena asphodeloides Bge.Polysaccharides and Their Effects on Brain Tissue and Gut Microbiota in Aging Mice
摘要
Abstract
This study characterized Anemarrhena asphodeloides Bge.polysaccharides(AABP)and evaluated their protective effects against D-galactose(D-gal)-induced brain injury and gut microbiota dysbiosis in aging mice.The results indicated that AABP,with a purity of 80.89%,were primarily composed of mannose and glucose,featured a triple-helix structure and possessed significant in vitro antioxidant capacity.AABP significantly improved the behavioral performance of aging mice and alleviated neuronal pathological damage in the hippocampus.Mechanistically,AABP effectively enhanced the activities of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)while reducing malondialdehyde(MDA)levels in brain tissue.Moreover,they activated the downstream protein kinase B(Akt)/cAMP-response element binding protein(CREB)signaling pathway by upregulating brain-derived neurotrophic factor(BDNF)expression.Furthermore,16S rRNA sequencing and functional prediction using PICRUSt2 revealed that AABP restored the α-diversity of the gut microbiota and the relative abundance of key genera,such as Lachnospiraceae_NK4A136_group and Alloprevotella,while enhancing carbohydrate and amino acid metabolism.In summary,AABP may exert anti-aging effects by activating the cerebral BDNF/Akt/CREB signaling pathway and remodeling gut microbiota homeostasis.关键词
知母多糖/结构表征/衰老小鼠/脑源性神经营养因子/蛋白激酶B/cAMP反应元件结合蛋白信号通路/肠道菌群Key words
Anemarrhena asphodeloides Bge.polysaccharides/structural characterization/aging mice/brain-derived neurotrophic factor/protein kinase B/cAMP-response element binding protein signaling pathway/gut microbiota分类
轻工纺织引用本文复制引用
孙婷婷,陈浩,俞浩,徐皓男,张放,吴玮,崔灿,袁小涌,崔雨,车燕,邵俊贤..知母多糖的制备、表征及其对衰老小鼠脑组织和肠道菌群的影响[J].食品科学,2026,47(9):190-202,13.基金项目
安徽省自然科学基金项目(2020085QH395) （2020085QH395）
安徽省药品监督管理局科技创新项目(AHYJ-KJ-202518) （AHYJ-KJ-202518）
安徽省高校中青年教师学科(专业)带头人培育项目(DTR2025033) （专业）
安徽省中医药传承创新研究项目(2024CCCX256) （2024CCCX256）
安徽省博士后科研资助项目(2024C853) （2024C853）
安徽省重点研究与开发计划项目(202004a07020031) （202004a07020031）
横向产学研合作项目(880598 （）
881383 （）
881384) （）
