食品科学2026，Vol.47Issue(10)：1-18,18.DOI:10.7506/spkx1002-6630-20260303-027
工程益生菌短链脂肪酸的合成生物学设计:从代谢强化到智能调控
Synthetic Biology Design of Engineered Probiotics for Short-Chain Fatty Acid Production:from Metabolic Enhancement to Smart Regulation
摘要
Abstract
Short-chain fatty acids(SCFAs),as key metabolites produced by the intestinal microbiota from the fermentation of dietary fiber and other substrates,play a crucial role in maintaining intestinal barrier function,regulating immune balance,and promoting systemic health.This review explores strategies for utilizing engineered food microorganisms(particularly probiotics)as novel"bio-factories"to achieve efficient and precise synthesis of SCFAs.This review outlines the core selection criteria for ideal engineered chassis strains,including their safety for application,robustness in the intestinal environment,and genetic tractability.It then details strategies for significantly enhancing the efficiency of SCFA synthesis in food microorganisms such as discovery and engineering of rate-limiting enzymes,intelligent enzyme engineering modifications,and artificial intelligence-driven metabolic network optimization.Furthermore,this review discusses how to integrate biosensors and clustered regularly interspaced short palindromic repeats-based dynamic regulation systems to achieve on-demand and precise modulation of SCFAs within the intestinal microecology.The application prospects of engineered food microorganisms are discussed in two major directions:First,in vitro food industrial biomanufacturing,i.e.,utilizing engineered strains to efficiently produce SCFAs in fermentation systems as food ingredients or additives;this pathway exhibits relatively high technical maturity and a relatively clear regulatory framework.Second,in vivo live biotherapeutics,i.e.,direct ingestion of engineered probiotics to in situ synthesize SCFAs in the gut.This pathway holds significant potential for personalized nutritional intervention and gut health management,but faces greater challenges in safety assessment and regulation.关键词
短链脂肪酸/工程益生菌/生物传感器/干扰型/激活型成簇规律间隔短回文重复序列/动态调控Key words
short-chain fatty acids/engineered probiotics/biosensors/clustered regularly interspaced short palindromic repeats interference/activation/dynamic regulation分类
轻工纺织引用本文复制引用
江凌,刘振磊,朱政明..工程益生菌短链脂肪酸的合成生物学设计:从代谢强化到智能调控[J].食品科学,2026,47(10):1-18,18.基金项目
国家自然科学基金面上项目(32472489) （32472489）
国家自然科学基金专项项目(32541093) （32541093）
江苏省合成生物基础研究中心项目(BK20233003) （BK20233003）
