食品科学2026，Vol.47Issue(10)：39-54,16.DOI:10.7506/spkx1002-6630-20251117-126
海南大叶茶特征多酚对碳水化合物水解酶的抑制机制
Inhibitory Mechanism of Characteristic Polyphenols in Hainan Large-Leaf Tea against Carbohydrate Hydrolases
摘要
Abstract
This study explored the inhibitory potential and mechanism of water extracts and characteristic polyphenols from green and black tea made from the Hainan large-leaf variety against carbohydrate-hydrolyzing enzymes(α-amylase and α-glucosidase).The results showed that green tea had significantly higher total phenolic(61.9 mg/g)and flavonoid(28.79 mg/g)contents and consequently exhibited stronger inhibition against α-glucosidase and α-amylase with half maximal inhibitory concentration(IC50)of 59.10 μg/mL and 14.21 mg/mL than black tea,respectively.Nine characteristic polyphenols were identified,and five core active monomers including epigallocatechin(EGC),epigallocatechin gallate(EGCG),procyanidin B2(PB2),kaempferol-3-O-rutinoside,and ellagic acid were screened out by molecular docking.These compounds demonstrated significant enzyme inhibitory effects and enhanced glucose consumption in 3T3-L1 cells.Mechanistic analysis revealed that the polyphenols inhibited the enzymes in competitive,non-competitive,and mixed-type manners.Fluorescence quenching and molecular simulations confirmed that the polyphenols could interact with the enzymes'active sites(ASP-300/GLU-233 in α-amylase;GLU-296/ASP-269 in α-glucosidase)via a static quenching process,and stable complexes were formed through hydrogen bonding and van der Waals forces,with EGCG-α-amylase and PB2-α-glucosidase complexes showing optimal stability.This study demonstrates that the characteristic polyphenols in Hainan large-leaf tea,particularly in green tea,regulate postprandial glucose through the dual pathways of enzyme inhibition and glucose consumption promotion.At their core,these polyphenols stably bind to the active sites of carbohydrate-hydrolyzing enzymes through multiple mechanisms.关键词
海南大叶茶/多酚/α-淀粉酶/α-葡萄糖苷酶/分子对接Key words
Hainan large-leaf tea/polyphenols/α-amylase/α-glucosidase/molecular docking分类
轻工纺织引用本文复制引用
刘孝泽,张淦淇,孙若梓莹,刘颖鑫,王露..海南大叶茶特征多酚对碳水化合物水解酶的抑制机制[J].食品科学,2026,47(10):39-54,16.基金项目
国家自然科学基金青年科学基金项目(32201991) （32201991）
国家自然科学基金地区科学基金项目(32360109) （32360109）
海南省农垦投资控股集团有限公司科技项目(HKZSJD202409) （HKZSJD202409）
海南省重点研发计划-市县联动项目(ZDYF2026SXLH055) （ZDYF2026SXLH055）
