食品与发酵工业2026，Vol.52Issue(10)：51-58,8.DOI:10.13995/j.cnki.11-1802/ts.043795
鼠李糖乳酪杆菌B6胞外多糖对脂多糖诱导Caco-2细胞损伤的影响
Effects of Lacticaseibacillus rhamnosus B6 exopolysaccharides on lipopolysaccharide-induced damage in Caco-2 cells
摘要
Abstract
To explore the protective effects and underlying mechanisms of Lactobacillus exopolysaccharides against lipopolysaccharide-induced intestinal barrier damage,a pure and structurally well-characterized polysaccharide(B6-EPS)isolated and purified from Lactica-seibacillus rhamnosus B6 was used as the research subject.The improvement effect of B6-EPS on LPS-induced injury in Caco-2 cell was systematically evaluated from multiple perspectives in vitro,including cell viability,inflammatory factor levels,transepithelial electrical resistance and permeability of Caco-2 cell monolayers,and tight junction protein expression.The results demonstrated that intervention with 200 μg/mL B6-EPS could significantly improve the viability of Caco-2 cells(P＜0.05),enhance the level of anti-inflammatory fac-tor IL-10(P＜0.01),and promote the expression of tight junction protein CLDN3 to improve the integrity of Caco-2 cell monolayers,thereby alleviating LPS-induced Caco-2 cell injury.In addition,the results of gene differential analysis also provided potential new targets for B6-EPS in regulating cellular inflammation and oxidative stress.Overall,the findings of this study provide scientific support for the pro-tective effect of probiotic exopolysaccharides against lipopolysaccharide-induced intestinal barrier damage,and lay a foundation for further investigations into the deep mechanisms by which CLDN3 mediates the regulation of intestinal barrier function.关键词
鼠李糖乳酪杆菌/胞外多糖/肠道屏障/紧密连接蛋白Key words
Lacticaseibacillus rhamnosus/exopolysaccharides/intestinal barrier/tight junction proteins引用本文复制引用
王丹其,胡丹,韩瑨..鼠李糖乳酪杆菌B6胞外多糖对脂多糖诱导Caco-2细胞损伤的影响[J].食品与发酵工业,2026,52(10):51-58,8.基金项目
十四五国家重点研发计划(2022YFD2100704) （2022YFD2100704）
上海市自然科学基金项目(23ZR1414400) （23ZR1414400）
