轻工学报2026，Vol.41Issue(3)：1-9,9.DOI:10.12187/2026.03.001
CRISPR/Cas12a-核酸等温扩增技术在食源性致病菌检测中的研究进展
Research advances of CRISPR/Cas12a-nucleic acid isothermal amplification technology in the detection of foodborne pathogens
摘要
Abstract
The CRISPR/Cas12a gene editing system can efficiently recognize and cleave exogenous nucleic acids,thus driving the innovation and development of nucleic acid-based detection technologies for foodborne pathogens.This review summarizes the research progress on integrating CRISPR/Cas 12a-based detection with various isothermal amplification techniques,including loop-mediated isothermal amplification(LAMP),recombinase polymerase amplification(RPA),recombinase-mediated amplification(RAA),rolling circle amplification(RCA),hybridization chain reaction(HCR),strand displacement amplification(SDA),and exponential amplification reaction(EXPAR),for the detection of foodborne pathogens.The combination of CRISPR/Cas12a with various isothermal amplification techniques enables highly specific and sensitive visual detection of foodborne pathogens,offering advantages such as simple operation,rapidity,and no requirement for complex instrumentation,while still facing limitations including high reagent costs,narrow temperature control range,relatively complex procedures,stringent primer design requirements,and low standardization.Future directions for the integration of CRISPR/Cas12a with isothermal amplification techniques focus on improving detection throughput and accuracy,developing automated and integrated platforms,and leveraging smartphone-based and artificial intelligence-based systems,thereby enabling"sample-in,result-out"one-pot on-site detection and providing technical support for more efficient,sensitive,simple,and rapid detection of foodborne pathogens.关键词
食源性致病菌/CRISPR/Cas12a/核酸等温扩增技术/基因检测Key words
foodborne pathogens/CRISPR/Cas12a/nucleic acid isothermal amplification/genetic detection分类
轻工纺织引用本文复制引用
胡金强,谢顺欣,贺雅玥,郭安,孙新城,董彩文,耿尧,高辉..CRISPR/Cas12a-核酸等温扩增技术在食源性致病菌检测中的研究进展[J].轻工学报,2026,41(3):1-9,9.基金项目
河南省科技攻关项目(242102321132) （242102321132）
河南省大学生创新训练计划项目(202410462037) （202410462037）
星空众创空间创新培育孵化项目(第一层次)(2020ZCKJ103) （第一层次）
