轻工学报2026，Vol.41Issue(3)：41-53,75,14.DOI:10.12187/2026.03.005
基于非靶向代谢组学的甘肃不同地区浆水代谢物差异分析
Analysis of metabolite differences in Jiang-shui from different regions based on untargeted metabolomics
摘要
Abstract
[Objective]This study aimed to investigate the metabolomic differences among Jiang-shui samples from different regions of Gansu Province.[Methods]Jiang-shui samples collected from Dingxi,Lanzhou,and Tianshui in Gansu Province were subjected to non-targeted metabolomics analysis using ultra-high performance liquid chromatography coupled with Orbitrap ExplorisTM 480 high-resolution mass spectrometry(UHPLC-Orbitrap ExplorisTM 480 MS)combined with multivariate statistical analysis.[Results]A total of 1746 metabolites were detected in the Jiang-shui samples from the three regions,with 1107 and 639 metabolites identified in positive and negative ion modes,respectively.These included 219 lipids and lipid-like molecules,96 phenylpropanoids and polyketides,446 organic heterocyclic compounds,360 organic acids and derivatives,284 benzenoids,183 organic oxygen compounds,49 organic nitrogen compounds,and 5 organic sulfur compounds.The Tianshui samples showed significant differences from those of the other two regions,whereas the Dingxi and Lanzhou samples exhibited certain similarities.Orthogonal partial least squares-discriminant analysis(OPLS-DA)with VIP>1 and P<0.05 identified 568 differential metabolites,including 36 taste-related metabolites.Among them,sour and bitter substances were the main contributors to the taste of Jiang-shui;sour substances were predominantly accumulated in Tianshui samples,while bitter substances were mainly concentrated in Lanzhou samples.Among the 21 bioactive substances identified,phenolic acids and organic acids were relatively abundant.Kyoto Encyclopedia of Genes and Genomes(KEGG)annotation revealed that the alanine,aspartate and glutamate metabolism pathway was significantly enriched among the differential metabolites from the three regions.[Conclusion]The Jiang-shui samples from different regions exhibited significant differences in metabolite composition,abundance,and metabolic pathways.These findings provide a reference for further understanding the quality formation mechanism of Gansu Jiang-shui.关键词
甘肃浆水/非靶向代谢组学/差异代谢物/生物活性物质/代谢通路Key words
Jiang-shui/non-targeted metabolomics/differential metabolite/biologically active substance/metabolic pathway分类
轻工纺织引用本文复制引用
杨文迪,张亚锋,汪月,文鹏程,张忠明,乔海军,张卫兵..基于非靶向代谢组学的甘肃不同地区浆水代谢物差异分析[J].轻工学报,2026,41(3):41-53,75,14.基金项目
甘肃省自然科学基金项目(24JRRA652,24JRRA634) （24JRRA652,24JRRA634）
国家自然科学基金项目(32460576) （32460576）
