粮油食品科技2026，Vol.34Issue(3)：53-63,11.DOI:10.16210/j.cnki.1007-7561.2026.03.007
不同蛋氨酸水平对人肠道菌群影响的体外评价研究
Effect of Diets with Different Methionine Levels on Gut Microbiota:An In Vitro Study
摘要
Abstract
Methionine is the only essential sulfur-containing amino acid in humans,and its intake varies considerably among populations with different dietary patterns;however,the impact of different methionine intake levels on the human gut microbiota remains unknown.This study aimed to investigate the effects of different methionine levels on the human gut microbiota using an in vitro simulated anaerobic fermentation method,in which 16S rDNA high-throughput sequencing was used to detect the composition of the gut microbiota,gas chromatography to determine the content of short-chain fatty acids(SCFAs),and methylene blue spectrophotometry to determine the content of hydrogen sulfide(H2S).The results showed that after in vitro fermentation,the fermentation broth was mainly composed of Firmicutes,Proteobacteria,and Bacteroidota at the phylum level,and at the genus level,with the increase in methionine level,the abundance of H2S-producing bacteria(Desulfovibrio and Bilophila)gradually increased,while the abundance of SCFA-producing bacteria(Lachnoclostridium,Bacteroides and Parabacteroides)gradually decreased,and the abundance of harmful bacteria(Escherichia-Shigella,Klebsiella,and Dialister)also gradually increased;in addition,the concentration of H2S in the fermentation broth increased gradually with the increase in methionine level,while the concentration of SCFAs decreased gradually,and correlation analysis revealed that H2S concentration was extremely significantly negatively correlated with SCFAs concentration,positively correlated with the abundance of harmful bacteria,and negatively correlated with the abundance of beneficial bacteria,while SCFAs concentration was negatively correlated with the abundance of harmful bacteria and positively correlated with the abundance of beneficial bacteria.Therefore,moderate reduction of methionine intake may have the potential to improve human intestinal microbiota composition and internal environment health,and this study provides a theoretical basis for establishing the association between dietary methionine intake and human intestinal homeostasis,as well as new scientific evidence for improving human gut microbiota and intestinal health through precision methionine nutrition.关键词
蛋氨酸/肠道菌群/硫化氢/短链脂肪酸/体外厌氧发酵Key words
methionine/hydrogen sulfide/short-chain fatty acids/gut microbiota/in vitro fermentation分类
轻工纺织引用本文复制引用
胡一龙,田振国,谢岩黎,杨玉辉..不同蛋氨酸水平对人肠道菌群影响的体外评价研究[J].粮油食品科技,2026,34(3):53-63,11.基金项目
国家自然科学基金青年基金项目"蛋氨酸限制饮食调控肠内H2S与丁酸互作改善肠粘膜屏障的分子机制"32502230 （）
中国博士后科学基金面上项目"可降低机体蛋氨酸水平的乳酸菌筛选及其肠道中降解机制研究"(2023M730977)National Natural Science Foundation of China"Molecular Mechanism Study on the Improvement of Dietary Methionine Restriction on Intestinal Mucosal Barrier by Regulating the Interaction Between Enteral H2S and Butyrate"(No.32502230) （2023M730977）
China Postdoctoral Science Foundation"Screening of Lactic Acid Bacteria that Reduce Methionine Levels in the Body and Study on Its Degradation Mechanism in the Gut"(No.2023M730977) （No.2023M730977）
