食品工业科技2026，Vol.47Issue(12)：27-36,10.DOI:10.13386/j.issn1002-0306.2025050293
汉麻仁肽对小鼠酒精性肝损伤的保护作用
Protective Effect of Hemp Peptide on Alcohol-induced Liver Injury in Mice
摘要
Abstract
To investigate the hepatoprotective effect of hemp peptide,an acute alcoholic liver injury model was established in C57BL/6 mice,and the mechanisms were examined with respect to lipid metabolism and oxidative stress.H&E and Oil Red O staining were performed to assess hepatic histopathology.Serum and liver biochemical parameters,antioxidant enzymes,and alcohol-metabolizing enzymes were measured using biochemical assay kits.Related lipid metabolism and oxidative stress proteins were analyzed by Western blot.The results showed that compared with the alcoholic liver injury model control group,the high-dose hemp peptide group(1300 mg/kg·bw)exhibited significantly decreased liver indices in mice(P<0.05),with the hemp peptide group showing the maximum reduction of 12.48%.In the high-dose hemp peptide group,serum levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),total cholesterol(TC),and triglycerides(TG)were significantly reduced respectively(P<0.05).The levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),and interleukin-8(IL-8)in serum were significantly reduced respectively(P<0.05).The activities of superoxide dismutase(SOD),glutathione(GSH),and glutathione peroxidase(GSH-Px)in liver tissue were significantly increased respectively,while the malondialdehyde(MDA)content was significantly decreased(P<0.05).Mechanistically,CYP2E1 protein expression was significantly inhibited by hemp peptide,resulting in enhanced alcohol metabolism.Mean-while,downregulation of SREBP-1c expression was observed,which effectively alleviated lipid metabolism disorders.Fur-thermore,PPARα protein expression was markedly upregulated,leading to accelerated fatty acid oxidation.Histopathologi-cal analysis revealed that hemp peptide ameliorated liver injury in mice to varying degrees.In conclusion,hemp peptide showed significant protective effects against alcohol-induced acute liver injury in mice,which would provide a theoretical foundation for developing hemp peptide functional foods.关键词
汉麻仁肽/急性酒精性肝损伤/氧化应激/脂质代谢/保护作用Key words
hemp peptide/acute alcohol-induced liver injury/oxidative stress/lipid metabolism/protective effect分类
轻工纺织引用本文复制引用
魏连会,董艳,王颖,张子怡,王丽卫,李国巍,吕文庆,李柏阳,韩舒晨,刘雨雯,董芷嘉..汉麻仁肽对小鼠酒精性肝损伤的保护作用[J].食品工业科技,2026,47(12):27-36,10.基金项目
黑龙江省省属科研院所科研业务费项目(CZKYF2024-1-B39) （CZKYF2024-1-B39）
黑龙江省科学院院所能力提升专项(YSTS2025DQ01) （YSTS2025DQ01）
江淮杂粮产业工程技术研发创新基地项目(2025024) （2025024）
大庆市指导性科技计划项目(zd-2024-48). （zd-2024-48）
