茶叶科学2026，Vol.46Issue(3)：499-508,10.
基于在线净化结合液相色谱-质谱技术识别茶叶中非法添加的9种合成色素
Identification of 9 Synthetic Pigments Illegally Added to Tea Based on Online Purification Combined with Liquid Chromatography-Mass Spectrometry Technology
摘要
Abstract
A liquid chromatography-tandem mass spectrometry method for the determination of nine synthetic pigments illegally added to tea,such as tartrazine and sunset yellow by online purification was established.The samples were first soaked in water,then extracted with ammonia water and methanol solution,purified online by Cyclone-P column,and then determined by liquid chromatography-tandem mass spectrometry(LC-MS/MS).Under optimized conditions,the nine pigments showed a good linear relationship within the mass concentration range of 0.010-1.0 μg·mL-1,with correlation coefficients greater than 0.999.The limits of quantitation(LOQ)of nine colorants were 0.10-0.50 mg·kg-1.The mean recoveries ranged from 71.5%to 105.9%,with the relative standard deviation of≤8.7%.This method is simple,rapid,has good selectivity and reproducibility,high sensitivity,accurate and reliable results.It has obvious advantages for the quantitative detection of illegal added pigments to tea samples.关键词
液相色谱-串联质谱/在线净化/茶叶/色素Key words
liquid chromatography-tandem mass spectrometry(LC-MS/MS)/online purification/tea/pigment分类
农业科技引用本文复制引用
黄超群,吴娟,陈红平,裘睿涵,黄佳波,王依,陈丽,韩超..基于在线净化结合液相色谱-质谱技术识别茶叶中非法添加的9种合成色素[J].茶叶科学,2026,46(3):499-508,10.基金项目
海关总署科研项目(2022HK123) （2022HK123）
