茶叶科学2026，Vol.46Issue(3)：535-544,10.
miR-146a介导的白茶提取物调控Ⅱ型肺泡细胞上皮-间质转化抑制干燥综合征肺纤维化的分子机制
Molecular Mechanism of White Tea Extract Regulating EMT in Type Ⅱ Alveolar Cells via miR-146a to Inhibit Pulmonary Fibrosis in Sjögren's Syndrome
摘要
Abstract
This paper aimed to investigate the mechanism of white tea extract on type Ⅱ alveolar cells and the expression level of microRNA-146a(miR-146a)in Sjogren's syndrome complicated with pulmonary fibrosis.Human alveolar epithelial A549 cells were divided into different groups:the blank group was not treated with transforming growth factor-β1(TGF-β1),the TGF-β1-induced group was treated with TGF-β1 without any intervention,the white tea extract group was treated with 50 μg·mL-1 white tea extract after TGF-β1 induction,the miR-146a mimic group was transfected with 6 μL miR-146a mimic after TGF-β1 induction;and the white tea extract+miR-146a mimic group was intervened with 50 μg·mL-1 white tea extract combined with 6 μL miR-146a mimic after TGF-β1 induction.Cell morphology was observed under a microscope and cell viability was detected by CCK-8 assay.The fluorescence intensities of E-cadherin and α-SMA proteins were measured by immunofluorescence.The expressions of SP-C and PDGFRα were detected by Western blotting.The expression levels of miR-146a,TGF-β1 and Smad2 were determined by qRT-PCR.The results show that compared with the blank group,the TGF-β1-induced group exhibited increased cell viability,fluorescence intensity of α-SMA protein,increased expressions of SP-C,PDGFRα and TGF-β1,as well as decreased expressions of E-cadherin and miR-146a(P<0.05).Compared with the TGF-β1-induced group,the white tea extract group and the miR-146a mimic group showed decreased cell viability,fluorescence intensity of α-SMA,expressions of SP-C,PDGFRα and TGF-β1,together with increased expressions of E-cadherin and miR-146a(P<0.05),with no statistically significant difference between the two groups(P>0.05).Compared with the miR-146a mimic group,the aforementioned indicators in the white tea extract+miR-146a mimic group changed more significantly(P<0.05).This study indicates that white tea extract can markedly inhibit the abnormal activation of A549 cells induced by TGF-β1,reverse the process of epithelial-mesenchymal transition(EMT)and downregulate fibroblast-related activity indicators.Its mechanism may be related to the up-regulation of miR-146a expression and the regulation of the TGF-β1/Smad2 signaling pathway.关键词
干燥综合征伴肺纤维化病变/白茶提取物/Ⅱ型肺泡细胞/转化生长因子-β1Key words
Sjögren's syndrome with pulmonary fibrosis/white tea extract/type Ⅱ alveolar cells/TGF-β1分类
农业科技引用本文复制引用
张晶晶,朱跃兰,王超..miR-146a介导的白茶提取物调控Ⅱ型肺泡细胞上皮-间质转化抑制干燥综合征肺纤维化的分子机制[J].茶叶科学,2026,46(3):535-544,10.基金项目
国家自然科学基金(81874438) （81874438）
