食用菌学报2026，Vol.33Issue(3)：33-41,9.DOI:10.16488/j.cnki.1005-9873.2026.03.004
铅胁迫对姬松茸菌丝生长和抗氧化系统的影响
Effects of Lead Stress on Mycelial Growth and Antioxidant System in Agaricus blazei
摘要
Abstract
The effects of lead stress to mycelia growth and antioxidant system of Agaricus blazei were studied by adding lead nitrate into the culture medium to Pb2+concentrations of 5,10,20,50,and 100 mg·L-1,respectively.Different Pb2+groups were determined for mycelial growth,lead content in mycelia and mycelial fermentation broth,contents of hydrogen peroxide(H2O2),malondialdehyde(MDA),ascorbic acid(ASA),reduced glutathione(GSH),and oxidized glutathione(GSSG)in mycelia,and activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),glutathione reductase(GR),glutathione peroxidase(GPX),and ascorbate peroxidase(APX)in mycelia.The results showed that lead stress inhibited mycelial growth.Both Pb2+content in mycelia and Pb2+concentration in the fermentation broth increased as the Pb2+concentration increased,peaking at(16.05±1.61)mg·kg-1 and(42.77±6.83)mg·L-1 under 100 mg·L-1 Pb2+,respectively.The contents of H2O2,MDA,and GSH,as well as the activities of SOD,CAT,POD,GR,and GPX in mycelia initially increased and then decreased.At 50 mg·L-1 Pb2+,the contents of H2O2 and MDA were relatively high,reaching(5.01±0.53)μmol·g-1 and(8.98±0.53)nmol·g-1,respectively.The GSH content and SOD activity under 50 mg·L-1 Pb2+reached the peak of(1 052.57±71.62)nmol·g-1 and(220.56±21.23)U·g-1,respectively.As the Pb2+concentration increased,both ASA content and APX activity continuously increased,reaching their highest levels at(4.70±0.08)μg·g-1 and(305.05±13.45)U·mg-1·min-1,respectively,when the Pb2+concentration was 100 mg·L-1.As the Pb2+concentration increased,mycelial GSSG content initially increased,followed with a decrease,and then increased again.It remained significantly higher than that of the control group,peaking at(104.19±8.02)nmol·g-1 under 20 mg·L-1 Pb2+.These results provided a reference for addressing Pb2+contamination in A.blazei cultivation.关键词
姬松茸/铅胁迫/菌丝/抗氧化系统Key words
Agaricus blazei/lead stress/mycelia/antioxidant system引用本文复制引用
马建帅,查磊,余昌霞,杨琳,郭倩,董沁,赵妍..铅胁迫对姬松茸菌丝生长和抗氧化系统的影响[J].食用菌学报,2026,33(3):33-41,9.基金项目
国家重点研发计划(2024YFD1200204) （2024YFD1200204）
