沈阳农业大学学报2026，Vol.57Issue(3)：40-52,13.DOI:10.3969/j.issn.1000-1700.2026.03.005
贝莱斯芽孢杆菌Apcs-1全基因组测序分析及对土壤微生物的影响
Whole-genome Sequencing Analysis of Bacillus velezensis Apcs-1 and Its Effects on Soil Microbial Community
摘要
Abstract
[Objective]In previoaus studies,our research group isolated a strain of Apcs-1 with significant antagonistic activity against potato common scab.[Method]To further elucidate its biocontrol mechanism,whole-genome sequencing and analysis of strain Apcs-1 were performed in this study,and the effects of this biocontrol bacterium on soil microbial communities were also determined.[Results]The results showed that the complete genome of strain Apcs-1 is 3 929 735 bp in length with a GC content of 46.50%,and harbors multiple functional genes related to biocontrol traits such as antibiosis,growth promotion and induced resistance.AntiSMASH analysis predicted abundant biosynthetic gene clusters encoding lipopeptides,polyketides,siderophores and other secondary metabolites,indicating a strong potential to produce diverse antimicrobial substances.Prediction using the VFDB database revealed that the strain carries defensive virulence factors,nonspecific virulence factors,aggressive virulence factors,and virulence-related regulatory genes.Further analysis of rhizosphere soil microbial communities after application of the strain fermentation broth demonstrated that B.velezensis Apcs-1 significantly increased the abundance and community evenness of beneficial rhizosphere.microorganisms(e.g.,phylum Actinobacteria,class Bacilli),and significantly enriched genes associated with key metabolic pathways including amino acid metabolism and carbohydrate metabolism in soil.[Conclusion]This study reveals the genetic basis and biocontrol functions of strain Apcs-1 at the genomic level and from the perspective of microbial community effects,providing a theoretical foundation for the development and application of this strain in the green control of potato common scab.关键词
马铃薯疮痂病/贝莱斯芽孢杆菌/全基因组/土壤微生物群落Key words
potato common scab/Bacillus velezensis/whole-genome sequencing/soil microbial community分类
农业科技引用本文复制引用
王凤鹭,魏玮,王艳平,贾景丽,李诗瑶,石玉立,张崇,贾立君,吴元华..贝莱斯芽孢杆菌Apcs-1全基因组测序分析及对土壤微生物的影响[J].沈阳农业大学学报,2026,57(3):40-52,13.基金项目
辽宁省科技重大专项项目(2025JH1/11700019) （2025JH1/11700019）
