食用菌学报2026，Vol.33Issue(3)：66-74,9.DOI:10.16488/j.cnki.1005-9873.2026.03.007
大白桩菇多酚提取工艺优化及抗氧化和抑制癌细胞增殖活性
Optimization of Polyphenol Extraction from Leucopaxillus giganteus and Evaluation of Antioxidant and Antiproliferative Activities
摘要
Abstract
Based on single-factor experiments,three factors-ethanol volume fraction,solid-liquid ratio,and ultrasonic time-were selected to optimize the polyphenol extraction process from Leucopaxillus giganteus using response surface methodology.The antioxidant activity of the obtained polyphenol extract was evaluated by determining its DPPH radical scavenging rate,ferric reducing antioxidant power(FRAP),and its inhibitory rate on the proliferation of T24 human bladder cancer cells in vitro.The results showed that the optimal extraction conditions were as follows:60％ethanol volume fraction,solid-liquid ratio of 1∶15 g·mL-1,and ultrasonic time 30 min.Under these conditions,the polyphenol content reached 6.383 μg·mL-1.Within the experimental range,the polyphenol extract showed the highest DPPH free radical scavenging rate of 94.21％and the maximum FRAP value of 2.14 mmol·100 g-1 at 50 mg·mL-1.The IC50 of the polyphenol extract against T24 cells was 0.274 mg·mL-1.These findings provided a reference for further development of Leucopaxillus giganteus.关键词
大白桩菇/多酚/响应面优化/抗氧化活性/膀胱癌T24细胞Key words
Leucopaxillus giganteus/polyphenols/response surface optimization/antioxidant activity/T24 human bladder cancer cell line引用本文复制引用
张雅君,古丽拜尔·艾赛提,李艳婷,郭尚..大白桩菇多酚提取工艺优化及抗氧化和抑制癌细胞增殖活性[J].食用菌学报,2026,33(3):66-74,9.基金项目
山西省基础研究计划青年科学研究项目(202403021222095、202103021223139) （202403021222095、202103021223139）
