食品与发酵工业2026，Vol.52Issue(11)：36-44,9.DOI:10.13995/j.cnki.11-1802/ts.044087
转座子技术在梭菌基因功能研究与遗传改造中的应用
Application of transposon technology in gene function research and genetic engineering of Clostridium
摘要
Abstract
Clostridium is a group of Gram-positive anaerobic bacteria widely distributed in natural environments and living organisms,playing a significant role in human health,biomedicine,and industrial production.However,due to the relatively limited molecular genet-ic tools available for Clostridium,the exploration of key functional genes,elucidation of molecular mechanisms,and the construction of high-performance engineered strains remain challenging.In recent years,transposon technology has emerged as an efficient genetic manip-ulation tool in research on Clostridium species,providing effective technical means for gene delivery,genome editing,functional genom-ics,and metabolic optimization.This article systematically reviews recent advances in transposon-related technologies for Clostridium,dis-cusses their current applications and potential in gene function identification and strain engineering,and offers perspectives on future tech-nological developments.The aim is to provide insights and references for both fundamental research and industrial applications of Clostridi-um.关键词
转座子技术/梭菌/基因功能/遗传改造Key words
transposon-related technologies/Clostridium/gene function/genetic modification引用本文复制引用
黄正,张智怡,叶伟,顾阳..转座子技术在梭菌基因功能研究与遗传改造中的应用[J].食品与发酵工业,2026,52(11):36-44,9.基金项目
江苏省前沿技术研发计划项目(BF2024079) （BF2024079）
上海市科学技术委员会科研计划项目(25ZR1401262,24HC2820800) （25ZR1401262,24HC2820800）
