食品与发酵工业2026，Vol.52Issue(11)：53-64,12.DOI:10.13995/j.cnki.11-1802/ts.044052
植物乳植杆菌FS5-5发酵葛根提取液解酒护肝功效与机制的研究
Mechanisms and efficacy of Lactiplantibacillus plantarum FS5-5 fermented Pueraria lobata extract in alcohol detoxification and liver protection
摘要
Abstract
The global health burden of alcohol-induced liver injury is increasing,necessitating effective hepatoprotective strategies.This study employs Lactiplantibacillus plantarum FS5-5 as a fermenting agent to bioferment Pueraria lobata extract.The optimization of fer-mentation parameters resulted in the production of a fermented product,fermented pueraria lobata extract by Lactiplantibacillus plantarum FS5-5(FPE5).The results indicate that fermentation significantly enhances the flavonoid content,total phenolic compounds,and antioxi-dant capacity of FPE5.Animal studies demonstrate,a high dose of FPE5(FH)demonstrated significant efficacy.FH prolonged the time to the loss of righting reflex,shortened the recovery time,and lowered the serum ethanol concentration.FH effectively reduces serum alanine aminotransferase and aspartate aminotransferase activities,ameliorates hepatic histopathological damage,while concurrently enhancing he-patic alcohol dehydrogenase and aldehyde dehydrogenase activities.It also elevates total superoxide dismutase activity,reduces the content of malondialdehyde,increases the content of glutathione,and suppresses pro-inflammatory cytokine expression.Mechanistic investigations indicate that FPE5 mitigates oxidative stress by downregulating Cyp2e1 gene expression and modulating the Keap1/Nrf2/HO-1 signaling pathway,thereby exerting hepatoprotective and anti-alcoholic effects.This study confirms that fermentation with Lactiplantibacillus planta-rum can significantly enhance the antioxidant capacity and hepatoprotective effects of Pueraria lobata,providing a theoretical foundation for the development of functional products aimed at alcohol detoxification and liver protection.关键词
植物乳植杆菌/葛根/发酵工艺/解酒护肝/酒精性肝损伤Key words
Lactiplantibacillus plantarum/Pueraria lobata/fermentation process/alcohol detoxification and hepatoprotection/alcoholic liv-er injury引用本文复制引用
杨海鑫,臧健,李彤,武俊瑞,夏苏宁,安飞宇,史海粟,郭佳,薛瑞霞,乌日娜..植物乳植杆菌FS5-5发酵葛根提取液解酒护肝功效与机制的研究[J].食品与发酵工业,2026,52(11):53-64,12.基金项目
国家自然科学基金面上项目(32172279) （32172279）
辽宁省"兴辽英才计划"项目(XLYC2213026,XLYC2402005) （XLYC2213026,XLYC2402005）
辽宁省科学技术计划项目(2024JH2/101900005) （2024JH2/101900005）
沈阳农业大学引进人才(高层次)科研启动费项目(2023YJRC002) （高层次）
沈阳市科技创新平台项目(21-103-0-14,21-104-0-28) （21-103-0-14,21-104-0-28）
