食品与发酵工业2026，Vol.52Issue(11)：116-123,8.DOI:10.13995/j.cnki.11-1802/ts.044415
传统筛选与常压室温等离子体诱变接续选育高产吡嗪类物质菌株及应用
Continuous breeding of high-yield pyrazine-producing strains via traditional screening and ARTP mutagenesis and their applications
摘要
Abstract
The integration of conventional screening techniques and ARTP mutagenesis presents a prominent advantage for strain im-provement in the Baijiu fermentation industry.This study focused on various Daqu samples from Sichuan-producing regions.Through a com-bined approach of traditional screening and ARTP mutagenesis,high-yield pyrazine-producing strains were successfully bred and systemat-ically characterized.Their practical application in actual Baijiu fermentation using fermented grains was further investigated.Results showed that the initially isolated strain X-23 was identified as Bacillus subtilis via molecular biology methods-a Gram-positive bacterium capable of producing 85.48 mg/kg of pyrazines.After ARTP mutagenesis,the mutant strain M-6 exhibited significant morphological chan-ges under scanning electron microscopy and showed enhanced protein metabolism capabilities.Specifically,gelatin liquefaction and mal-onate test results turned from negative to positive.Under solid-state sorghum culture conditions,the total volatile compound production by M-6 increased by 140.26％compared to the wild strain,with pyrazine yield reaching 1 240.36 mg/kg-14.51 times higher than that of the original strain.The mutant also produced a more diverse profile of pyrazine compounds.Fermentation validation in actual grain compost further confirmed that inoculation with M-6 led to the detection of dimethyl-pyrazine[(21.37±2.82)mg/kg],trimethyl-pyrazine[(27.23±1.95)mg/kg],and tetramethyl-pyrazine[(60.71±1.42)mg/kg].Thus,M-6 demonstrates excellent pyrazine synthesis ca-pacity in fermented grain systems.This study provides novel microbial resources and a theoretical foundation for enhancing product quality and innovating brewing processes in China's traditional Baijiu industry.关键词
白酒/吡嗪类物质/ARTP技术/自然筛选/糟醅Key words
Baijiu/pyrazines/ARTP/natural screening/zaopei引用本文复制引用
陈晓茹,刘林培,郑佳,尹礼国,林涵瑞,伍升杰,魏明英,隋明,唐贤华,张崇军..传统筛选与常压室温等离子体诱变接续选育高产吡嗪类物质菌株及应用[J].食品与发酵工业,2026,52(11):116-123,8.基金项目
四川省科技计划苗子工程重点项目(2024JDRC0028) （2024JDRC0028）
固态发酵资源利用四川省重点实验室开放基金项目(2024GTJJ04) （2024GTJJ04）
四川工商职业技术学院青年专项课题(2024NC08) （2024NC08）
