现代食品科技2026，Vol.42Issue(5)：1-12,12.DOI:10.13982/j.mfst.1673-9078.2026.5.0316
石斛碱修复Caco-2+HT29-MTX/RAW 264.7细胞肠屏障并改善HepG2细胞胰岛素抵抗的双重作用
Dual Effects of Dendrobine on Intestinal Barrier Repair in Caco-2+HT29-MTX/RAW 264.7 Cells and Insulin Resistance Improvement in HepG2 Cells
摘要
Abstract
To investigate the effects of dendrobine(DEN)on intestinal barrier repair and insulin resistance improvement,a Caco-2+HT29-MTX/RAW 264.7 co-culture intestinal barrier model and an insulin-resistant HepG2 cell model were established.Barrier function and inflammatory responses were evaluated using transepithelial electrical resistance(TEER),real-time quantitative PCR,and western blot analysis,while glucose metabolism-related indicators were assessed in the insulin resistance model.The results showed that,compared with the model group,DEN maintained TEER values above 200 Ω·cm2 and significantly upregulated the protein expression of tight junction components claudin-1(72.19％),occludin(82.40％),ZO-1(75.29％),and mucin MUC2(70.33％).Correspondingly,mRNA expression levels increased by 1.47％,452.93％,2.11％,and 10.38％,respectively.In addition,DEN significantly reduced the levels of pro-inflammatory cytokines TNF-α(14.14％)and IL-6(68.93％),while markedly increasing the levels of anti-inflammatory cytokines IL-4(243.96％)and IL-10(41.35％).In the insulin-resistant HepG2 cell model,DEN at 100,200,and 300 ng·mL-1 increased glucose uptake in a dose-dependent manner(10.70,11.83,and 13.04 mmol·L-1,respectively).DEN also enhanced the activities of pyruvate kinase(92.14,107.09,and 122.02 U·g-1 protein)and hexokinase(29.63,73.99,and 73.84 U·g-1 protein),and increased intracellular glycogen content by 1.2-,1.4-,and 1.5-fold,respectively.Overall,DEN effectively repaired intestinal barrier dysfunction and significantly improved insulin resistance.These findings provide a theoretical basis for the development of DEN-based functional foods or therapeutic agents targeting hyperglycemia and metabolic disorders.关键词
石斛碱/Caco-2+HT29-MTX/RAW 264.7/HepG2/肠道屏障/胰岛素抵抗Key words
dendrobine/Caco-2+HT29-MTX/RAW 264.7 cells/HepG2 cells/intestinal barrier/insulin resistance引用本文复制引用
覃璐琪,周怡霞,范蓓,孙晶,卢聪,刘佳萌,王凤忠..石斛碱修复Caco-2+HT29-MTX/RAW 264.7细胞肠屏障并改善HepG2细胞胰岛素抵抗的双重作用[J].现代食品科技,2026,42(5):1-12,12.基金项目
国家重点研发计划项目(2022YFD1600303) （2022YFD1600303）
云南省重大科技专项(202402AE090011) （202402AE090011）
