现代食品科技2026，Vol.42Issue(5)：158-166,9.DOI:10.13982/j.mfst.1673-9078.2026.5.0256
乙酰化霍山石斛多糖制备工艺及其抗氧化抗肿瘤活性分析
Preparation of Acetylated Dendrobium huoshanense Polysaccharides and Analysis of Antioxidant and Antitumor Activities
摘要
Abstract
To investigate the effects of acetylation modification on the biological activities of Dendrobium huoshanense polysaccharides(DHP),acetylated D.huoshanense polysaccharides(A-DHP)were prepared using the acetic anhydride method,and the preparation process was optimized.The degree of substitution was used as the evaluation index,and synthesis conditions were optimized using single-factor experiments and response surface methodology.The structure of A-DHP was characterized using Fourier transform infrared spectroscopy(FT-IR)and scanning electron microscopy(SEM).Changes in antioxidant and antitumor activities before and after acetylation were evaluated using in vitro antioxidant essays and cytotoxicity experiments.The results showed that the optimal preparation conditions were a reaction time of 3.8 h,an acetic anhydride-to-polysaccharide ratio of 14∶1(mL∶g),and a reaction temperature of 36 ℃.Under these conditions,the degree of substitution of A-DHP reached 0.226.FTIR and SEM analyses confirmed the successful introduction of acetyl groups into the DHP structure.In vitro antioxidant assays demonstrated that acetylation increased the scavenging activities against ABTS and DPPH free radicals by 14.91％and 7.76％,respectively.In addition,cytotoxicity assays showed that the inhibitory effects of A-DHP on HepG2 and C918 cells increased by 15.90％and 16.24％,respectively.Overall,acetylation modification effectively enhanced the antioxidant and antitumor activities of DHP.These findings provide a theoretical basis for the further development and application of acetylated polysaccharides.关键词
霍山石斛/多糖/乙酰化/抗氧化活性/抗肿瘤活性Key words
Dendrobium huoshanense/polysaccharides/acetylation/antioxidant activity/antitumor activity引用本文复制引用
纪俊豪,刘东,高雷雷,宋露,杨素素,王芳,王威,李国四,易善勇,王成,韩邦兴..乙酰化霍山石斛多糖制备工艺及其抗氧化抗肿瘤活性分析[J].现代食品科技,2026,42(5):158-166,9.基金项目
国家重点研发计划项目(2023YFC3503804) （2023YFC3503804）
财政部和农业农村部国家现代农业产业技术体系项目(CARS-21) （CARS-21）
安徽省现代中药产业共性技术研究中心开放课题(AHTCMGTRC-2023-03) （AHTCMGTRC-2023-03）
