乳业科学与技术2026，Vol.49Issue(3)：17-24,8.DOI:10.7506/rykxyjs1671-5187-20260220-014
长双歧杆菌长亚种BD3150对肠出血性大肠杆菌O157:H7活性的抑制作用
Inhibitory Effect of Bifidobacterium longum subsp.longum BD3150 on Enterohemorrhagic Escherichia coli O157:H7
摘要
Abstract
This study systematically investigated the inhibitory activity of Bifidobacterium longum subsp.longum BD3150 and its cell-free supernatant(CFS)against enterohemorrhagic Escherichia coli O157:H7(EHEC O157)using the co-culture assay and the agar diffusion method.When 2'-fucosyllactose(2'-FL)served as the sole carbon source,the relative abundance of EHEC O157 after 6 and 18 h of co-culture with B.longum subsp.longum BD3150 was 72.3％and 67.8％,respectively,which was lower than that observed using glucose as the sole carbon source.The antibacterial activity of CFS from B.longum subsp.longum BD3150,cultivated in modified de Man-Rogosa-Sharpe(MRS)medium,was further characterized.The optimum culture conditions were found to be an inoculation size of 3.0％,incubation temperature of 37 ℃,and incubation time of 36 h.A negative correlation was observed between the diameter of the inhibition zone and the pH of the CFS.Furthermore,by adjusting the pH of the CFS and performing centrifugal ultrafiltration(3 kDa,7 500 × g for 25 min),it was revealed that the inhibitory activity against EHEC O157 was attributed to organic acids.关键词
双歧杆菌/抑菌活性/琼脂扩散法/无细胞上清液/母乳寡糖Key words
Bifidobacterium/antibacterial activity/agar diffusion method/cell free supernatant/human milk oligosaccharides分类
医药卫生引用本文复制引用
王禕天,王晶,任全路,吴正钧..长双歧杆菌长亚种BD3150对肠出血性大肠杆菌O157:H7活性的抑制作用[J].乳业科学与技术,2026,49(3):17-24,8.基金项目
"十四五"国家重点研发计划重点专项(2022YFD2100705) （2022YFD2100705）
上海市国资委企业创新发展与能级提升项目(2022013) （2022013）
