食品科学2026，Vol.47Issue(11)：289-297,9.DOI:10.7506/spkx1002-6630-20251127-216
基于蛋白质组学解析马铃薯应对低O2高CO2胁迫的响应机制
Proteomic Analysis of the Response Mechanism of Potatoes to Low O2 and High CO2 Stress
摘要
Abstract
To elucidate the molecular mechanism by which low O2 and high CO2 environments regulate the postharvest storage quality of potatoes,the distribution characteristics and functions of differentially expressed proteins(DEPs)in'Atlantic'potatoes at the midpoint(day 60)and end(day 120)of storage were analyzed using data-independent acquisition(DIA)proteomics combined with subcellular localization prediction.The results showed that a total of 1 312 DEPs were induced in potatoes under low O2 and high CO2 stress,primarily enriched in organelles such as the cytoplasm,chloroplasts,and endoplasmic reticulum.Functional enrichment analysis revealed that low O2 and high CO2 stress significantly affected pathways including protein processing in the endoplasmic reticulum,photosynthesis,phenylpropanoid biosynthesis,starch and sucrose metabolism,and the mitogen-activated protein kinase(MAPK)signaling pathway.Proteins including members of the HSP20 family,ferredoxin,peroxidase,calmodulin,β-fructofuranosidase,and α-amylase synergistically responded to low O2 and high CO2 stress,collectively regulating relevant metabolic pathways to effectively delay"low-temperature sweetening"(LTS)in potatoes.In conclusion,at the protein level,this study reveals the molecular mechanism by which low O2 and high CO2 treatment synergistically alleviate LTS of potato through multiple pathways,providing a theoretical basis for optimizing controlled atmosphere storage techniques for processed potatoes.关键词
马铃薯/低O2高CO2贮藏/胁迫响应/蛋白质组学/差异表达蛋白/亚细胞定位Key words
potato/low O2 and high CO2 storage/stress response/proteomics/differentially expressed proteins/subcellular localization分类
农业科技引用本文复制引用
田甲春,葛霞,李梅,李守强,张亚倩,程建新,田世龙,李玉梅..基于蛋白质组学解析马铃薯应对低O2高CO2胁迫的响应机制[J].食品科学,2026,47(11):289-297,9.基金项目
国家自然科学基金地区科学基金项目(32160596) （32160596）
财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-09-P26) （CARS-09-P26）
