食品科学2026，Vol.47Issue(12)：13-20,8.DOI:10.7506/spkx1002-6630-20251230-267
阿魏酸和树脂酚甲酸协同抑菌效果及机理
Synergistic Antibacterial Effect and Mechanism of Ferulic Acid and 2,4-Dihydroxybenzoic Acid
摘要
Abstract
To screen for promising antimicrobial agents from natural sources,this study evaluated the antibacterial effect of nine polyphenols against Escherichia coli based on minimum inhibitory concentration(MIC)and fractional inhibitory concentration index(FICI).From these,ferulic acid and 2,4-dihydroxybenzoic acid were selected for their synergistic antibacterial effect against E.coli.Scanning electron microscopy(SEM)and fluorescence microscopy(FM)were used to observe micro-morphological changes in bacterial cells before and after treatment with ferulic acid and 2,4-dihydroxybenzoic acid.Fluorescent probes were applied to determine membrane potential,inner and outer membrane permeability,and reactive oxygen species(ROS)levels.The effect on biofilm formation was investigated using crystal violet staining.The results indicated that the two phenolic acids exerted synergistic antibacterial effect primarily by reducing transmembrane potential,increasing membrane permeability,disrupting membrane integrity as well as bacterial morphology,significantly lowering intracellular ROS levels to interfere with redox signaling,and effectively inhibiting biofilm formation.This study demonstrated great potential for the combined application of these phenolic acids as natural antibacterial agents.关键词
阿魏酸/树脂酚甲酸/协同抑菌机理/大肠杆菌Key words
ferulic acid/2,4-dihydroxybenzoic acid/synergistic antibacterial mechanism/Escherichia coli分类
轻工纺织引用本文复制引用
付荣耀,黄澳,叶江平,罗舜菁,刘成梅..阿魏酸和树脂酚甲酸协同抑菌效果及机理[J].食品科学,2026,47(12):13-20,8.基金项目
江西省引进培养创新创业高层次人才"千人计划"-科技创新类高端人才项目(jxsq2023201042) （jxsq2023201042）
江西省中央引导地方科技发展资金项目(20252ZDD020002) （20252ZDD020002）
