中国酿造2026，Vol.45Issue(7)：17-25,9.DOI:10.11882/j.issn.0254-5071.2026.07.003
内蒙古驼奶源高血管紧张素转换酶抑制活性益生菌筛选及其发酵条件优化
Screening of Probiotics with High Angiotensin-Converting Enzyme Inhibitory Activity from Inner Mongolia Camel Milk and Optimization of Their Fermentation Conditions
摘要
Abstract
To screen probiotic strains with high angiotensin-converting enzyme(ACE)inhibitory activity,539 preserved probiotic strains in laboratory were used as research materials.Strains with strong ACE inhibitory activity were preliminarily screened according to ACE inhibition rate and further identified via physiological-biochemical tests and molecular biological methods.The fermentation conditions for the selected strain were optimized using single-factor experiments,Plackett-Burman design,steepest ascent experiment,and Box-Behnken design.The results showed that strain Y43-3-1,which exhibited the highest ACE inhibitory activity,was isolated and identified as Lactiplantibacillus plantarum.The optimal fermentation conditions were as follows:glucose 1.9 g/100 mL,yeast powder 12 g/L,fermentation duration 28 h,incubation temperature 37℃,inoculation volume 2%(V/V),initial pH 6.0.Under optimized conditions,the ACE inhibition rate reached 65.72%,which was significantly higher than the initial value of 58.16%.In summary,L.plantarum Y43-3-1 with potent ACE inhibitory activity was screened out,and its fermentation conditions were optimized in this study.This work provided excellent strain resources and theoretical support for its development and application in antihypertensive functional foods or probiotic preparations.关键词
血管紧张素转换酶/抑制活性/植物乳植杆菌/发酵/条件优化Key words
angiotensin-converting enzyme/inhibitory activity/Lactiplantibacillus plantarum/fermentation/condition optimization分类
轻工纺织引用本文复制引用
李建雯,胡慧娟,刘梦雪,王正,于靖和,乌云达来..内蒙古驼奶源高血管紧张素转换酶抑制活性益生菌筛选及其发酵条件优化[J].中国酿造,2026,45(7):17-25,9.基金项目
国家自然科学基金地区科学基金项目(32360570) （32360570）
2023年自治区重点研发和成果转化计划(科技支撑东北振兴)项目(2023YFDZ0046) （科技支撑东北振兴）
2023年度呼伦贝尔市"科技兴市"行动重点项目(2023HZZX011) （2023HZZX011）
2023年度呼伦贝尔市应用技术研究与开发资金项目(NC2023020) （NC2023020）
