包装与食品机械2026，Vol.44Issue(4)：80-88,9.DOI:10.3969/j.issn.1005-1295.2026.04.008
畜禽产品中甲氧苄啶残留检测的方法研究
Method development for the detection of trimethoprim residues in livestock and poultry products
王佳琪 1萨仁图雅 2谷永丽 1皇金龙 1包阿如那 1闫炜3
作者信息
- 1. 呼伦贝尔市农畜产品质量安全中心/呼伦贝尔市农牧业检验检测中心,内蒙古 呼伦贝尔 021008
- 2. 新巴尔虎右旗草原事业发展中心,内蒙古 呼伦贝尔 021309
- 3. 兴安盟现代畜牧业试验区创建服务中心,内蒙古 兴安盟 137000
- 折叠
摘要
Abstract
To improve the efficiency of trimethoprim(TMP)residue detection in livestock and poultry products,an analytical method was developed using high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)combined with pass-through solid-phase extraction(SPE)for the determination of TMP residues.Meat and egg samples were extracted with acidified acetonitrile-water,salted out with Na2SO4,and purified using EMR-Lipid SPE cartridges.Chromatographic separation was performed on a ZORBAX Eclipse Plus C18 column,and detection was carried out using electrospray ionization in positive ion mode under multiple reaction monitoring,with quantification by external standard method.The results demonstrated good linearity for TMP in the ranges of 1-20 μg/L for meat and 0.25-5.0 μg/L for eggs,with correlation coefficients larger than 0.99.The limits of detection(LOD)were 0.5 μg/kg for meat and 0.1 μg/kg for eggs,and the limits of quantification(LOQ)were 1.0 μg/kg for meat and 0.2 μg/kg for eggs.At low,medium,and high spiking levels,the recoveries of TMP ranged from 70%to 120%with relative standard deviations(RSD)≤15%in meat matrices,and from 60%to 120%with RSD≤20%in egg matrices.Compared with the standard method,statistical analysis and t-test showed P>0.05,indicating no statistically significant difference and confirming that the method meets the requirements for method equivalence validation.This study provides robust technical support for veterinary drug residue monitoring and food safety control.关键词
畜禽产品/高效液相色谱-串联质谱/甲氧苄啶Key words
livestock and poultry products/high-performance liquid chromatography-tandem mass spectrometry/trimethoprim分类
轻工纺织引用本文复制引用
王佳琪,萨仁图雅,谷永丽,皇金龙,包阿如那,闫炜..畜禽产品中甲氧苄啶残留检测的方法研究[J].包装与食品机械,2026,44(4):80-88,9.
