茶叶科学2026，Vol.46Issue(4)：693-707,15.
聚乙烯与镉胁迫下枯草芽孢杆菌与沼泽红假单胞菌对茶苗安全生长的影响
Effects of Bacillus subtilis and Rhodopseudomonas palustris on the Safe Growth of Tea Seedlings Under Combined PE and Cd Stress
摘要
Abstract
To elucidate the remediation potential and underlying mechanisms of a microbial composite agent against polyethylene(PE)microplastic and cadmium(Cd)co-contamination in tea garden soils,a pot experiment was conducted using Camellia sinensis cv.'Longjing 43'seedlings.Soil samples were spiked with PE particle suspension and CdCl2·5H2O to simulate co-contamination stress.A microbial composite agent composed of Bacillus subtilis and Rhodopseudomonas palustris with a 1:1 viable cell ratio was subsequently applied to explore the effects of different application concentrations on soil biological activity,microbial community structure,as well as tea seedling morphology,Cd accumulation,stress resistance,photosynthetic parameters,and related gene expression.The results show that the application of the composite agent significantly enhanced soil enzyme activities,promoted the immobilization of available Cd,optimized the microbial community structure,and increased the abundance of beneficial microbial groups.At the plant level,the agent reduced Cd translocation to tea leaves,alleviated oxidative damage,and restored photosynthetic performance and antioxidant defense in tea seedlings.Expressions of genes related to α-linolenic acid metabolism,phenylalanine metabolism,and fatty acid catabolism were up-regulated,indicating the activation of stress-adaptive metabolic pathways.These findings indicate that the microbial composite agent can effectively mitigate oxidative damage and heavy metal toxicity induced by PE-exacerbated Cd stress via regulating the soil environment and the physiological and molecular networks in tea plants in a concentration-dependent manner.This study provided a theoretical basis and technical support for the bioremediation of microplastic and heavy metal co-contamination in tea garden soils and for the safe production of tea plants.关键词
茶园/复合菌剂/镉污染/微塑料/作用机制Key words
tea garden/composite microbial agent/Cd contamination/microplastic-mediated/mechanism of action分类
农业科技引用本文复制引用
赵银雪,孙小红,张意卓,杨敏成,官妍丹,侯琳,尼芳源,王凯林,王国夫,吴丽元..聚乙烯与镉胁迫下枯草芽孢杆菌与沼泽红假单胞菌对茶苗安全生长的影响[J].茶叶科学,2026,46(4):693-707,15.基金项目
国家自然科学基金(32101669) （32101669）
浙江省自然科学基金(LQ19C160003) （LQ19C160003）
绍兴市科技强农专项(2024A12003) （2024A12003）
