食品科学2026，Vol.47Issue(14)：94-104,11.DOI:10.7506/spkx1002-6630-20260105-020
黄皮叶精油对变形链球菌的抑制作用及其机制
Inhibitory Effect and Mechanism of Wampee Leaf Essential Oil against Streptococcus mutans
摘要
Abstract
In this study,essential oil(WLEO)from wampee leaves was extracted by steam distillation,and its chemical composition was analyzed using gas chromatography-mass spectrometry(GC-MS).The results showed that WLEO was mainly composed of sesquiterpene alcohols and sesquiterpene hydrocarbons.Subsequently,its inhibitory effects and underlying mechanisms against Streptococcus mutans and its biofilms were systematically evaluated.It was demonstrated that WLEO exhibited significant inhibitory effects against S.mutans,with an inhibition zone diameter of(22.33±0.74)mm.The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)were determined to be 2.5 and 5 mg/mL,respectively.The bacterial growth curve further confirmed that WLEO effectively inhibited bacteria growth at the MIC.Crystal violet staining and XTT assays showed that WLEO significantly reduced the total biomass and metabolic activity of S.mutans biofilms.Furthermore,WLEO suppressed biofilm formation by inhibiting extracellular polysaccharide synthesis.Membrane potential analysis and live/dead fluorescence staining revealed that WLEO disrupted the integrity of the bacterial cell membrane,resulting in the leakage of intracellular components.Untargeted metabolomics analysis further revealed that WLEO exerted its antibacterial activity by interfering with lipid metabolism,energy metabolism and other related metabolic pathways.Collectively,these results suggest that WLEO inhibits the growth and biofilm formation of S.mutans through a synergistic multitarget mechanism,providing a theoretical basis for its potential application in the food and related fields.关键词
黄皮叶精油/变形链球菌/生物膜/抑菌机制Key words
wampee leaf essential oil/Streptococcus mutans/biofilm/antibacterial mechanism分类
轻工纺织引用本文复制引用
曹世浩,李婕,张宇航,陈惠琴,朱婷婷..黄皮叶精油对变形链球菌的抑制作用及其机制[J].食品科学,2026,47(14):94-104,11.基金项目
海南省研究生创新科研课题(Qhys2023-513) （Qhys2023-513）
海南热带海洋学院硕士研究生创新项目(RHDYC-202420) （RHDYC-202420）
海南省柔性引才协同创新中心项目 （）
