食品与发酵工业2026，Vol.52Issue(15)：29-37,9.DOI:10.13995/j.cnki.11-1802/ts.045998
植物乳植杆菌F44的抑菌特性及其细菌素基因簇的挖掘
Antibacterial properties and mining of bacteriocin gene clusters of Lactiplantibacillus plantarum F44
摘要
Abstract
This study aimed to screen lactic acid bacteria that have an inhibitory effect on the dominant spoilage bacterium of chilled chicken,Pseudomonas lundensis,analyze their antibacterial characteristics,and mine potential bacteriocin gene clusters based on whole-genome sequences,ultimately evaluating their effectiveness in preserving chilled chicken.The results indicated that the selected Lactipla-ntibacillus plantarum F44 had a significant inhibitory effect on P.lundensis,with a minimum inhibitory concentration value of 312 μg/mL.The crude bacteriocin extract was sensitive to proteases,remained highly active against bacteria at 40-121 ℃ and pH range of 2.0-10.0,and exhibited antibacterial activity against common Gram-positive and Gram-negative bacteria.Whole-genome analysis showed that strain L.plantarum F44 had a genome length of 3 746 814 bp,a GC content of 41.33％,and contained five bacteriocin gene clusters.Af-ter treatment of chilled chicken with F44 bacteriocin(2MIC),the total bacterial count decreased by 2.6 lg CFU/g and the thiobarbituric acid reactive substances content decreased by 0.35 mg/kg after 12 days of refrigeration,extending the shelf life by 3 days.In summary,L.plantarum F44 and the bacteriocins it produces have good antibacterial properties and preservation potential,providing new microbial resources and a theoretical basis for the development of natural preservatives,and have promising application prospects.关键词
植物乳植杆菌/全基因组测序/隆德假单胞菌/细菌素Key words
Lactiplantibacillus plantarum/whole genome sequencing/Pseudomonas lundensis/bacteriocin引用本文复制引用
白越,孙芝兰,王坤,刘芳..植物乳植杆菌F44的抑菌特性及其细菌素基因簇的挖掘[J].食品与发酵工业,2026,52(15):29-37,9.基金项目
国家重点研发计划项目(2025YFF1107403) （2025YFF1107403）
