食品与发酵工业2026，Vol.52Issue(15)：46-54,9.DOI:10.13995/j.cnki.11-1802/ts.046382
胆盐胁迫下副溶血性弧菌生物被膜形成的异质性
Heterogeneity of biofilm formation in Vibrio parahaemolyticus under bile salt stress
摘要
Abstract
To accurately investigate the heterogeneity of biofilm formation by Vibrio parahaemolyticus under various concentrations of bile salt stress,this study selected six strains isolated from seawater,freshwater,and clinical samples.Crystal violet staining and the Cell Counting Kit-8(CCK-8)assay were employed to determine the differences in biofilm-forming capacity and cell viability under varying bile salt concentrations.Additionally,confocal laser scanning microscopy(CLSM)was utilized to observe the biofilm architecture and analyze changes in biofilm biomass and structural parameters under bile salt stress.The results indicated that bile salts inhibited biofilm formation in both environmental and clinical V.parahaemolyticus strains,leading to a significant reduction in biofilm biomass and cell viability,while delaying the time required to reach peak biofilm mass.Under high-concentration bile salt stress,clinical strains exhibited greater bio-film-forming capacity than environmental strains;however,under low-concentration bile salt stress,environmental strains outperformed clinical strains in biofilm formation.Furthermore,strains containing the tdh gene demonstrated a rapid response to and stronger tolerance against bile salt stress.These findings reveal the heterogeneity in the biofilm formation process and the time to reach maximum biofilm bio-mass among V.parahaemolyticus strains under varying bile salt stress levels,providing novel insights for the safety control of V.parahaemo-lyticus and targeted interventions for host intestinal infections.关键词
胆盐/胁迫/副溶血性弧菌/生物被膜/异质性Key words
bile salt/stress/Vibrio parahaemolyticus/biofilm/heterogeneity引用本文复制引用
赵伟,张洲,刘海泉,刘靖宇,赵勇,孟俊龙..胆盐胁迫下副溶血性弧菌生物被膜形成的异质性[J].食品与发酵工业,2026,52(15):46-54,9.基金项目
2025年度三晋英才计划自然科学和工程技术领域创新团队资助(SJYC2025538) （SJYC2025538）
国家自然科学基金项目(31671779) （31671779）
