食品与发酵工业2026，Vol.52Issue(15)：55-62,8.DOI:10.13995/j.cnki.11-1802/ts.044428
酵母浸粉在温度敏感型谷氨酸棒杆菌种子培养中的应用研究
Application of yeast extract in seed culture of temperature-sensitive Corynebacterium glutamicum
摘要
Abstract
In industrial glutamic acid production,Corynebacterium glutamicum temperature-sensitive strains serve as the primary mi-crobial hosts.Conventional nitrogen sources like soybean meal hydrolysate or corn steep liquor have been used during seed culture.Howev-er,inconsistent seed quality and frequent contamination often reduced production stability.This study evaluated the feasibility of replacing these nitrogen sources with yeast extract in the seed culture of temperature-sensitive C.glutamicum.Through spent medium analysis,the requirements for ammonium,peptides of varying molecular weights,and specific amino acids during bacterial growth were assessed.Differ-ent nitrogen sources were compared for their effects on growth rates.Key limiting nutrients were identified,leading to the selection of a suitable yeast extract.Results showed that serine,cystine,histidine,and isoleucine acted as limiting amino acids.Yeast extract FM308,rich in these amino acids,supported effective seed culture at an optimal concentration of 2 g/L.This approach shows significant potential for improving seed quality and reducing contamination in industrial glutamic acid fermentation.关键词
酵母浸粉/温度敏感型谷氨酸棒杆菌/氨基酸/培养基消耗分析Key words
yeast extract/temperature-sensitive Corynebacterium glutamicum/amino acids/spent media analysis引用本文复制引用
丁永志,刘登辉,胡悦,卢慧芳,伍业旭,李欣..酵母浸粉在温度敏感型谷氨酸棒杆菌种子培养中的应用研究[J].食品与发酵工业,2026,52(15):55-62,8.基金项目
湖北省自然科学基金计划项目(2024AFD152) （2024AFD152）
