食品与发酵工业2026，Vol.52Issue(15)：63-71,9.DOI:10.13995/j.cnki.11-1802/ts.044812
树莓低聚糖ERP-1结构表征及体外免疫调节活性研究
Structural characterization and in vitro immunomodulatory activity of raspberry-derived oligosaccharide ERP-1
摘要
Abstract
In this study,an oligosaccharide named ERP-1 was isolated from raspberry(Rubus idaeus L.)fruits.Its chemical struc-ture was elucidated,and in vitro immunomodulatory activity was evaluated.Crude polysaccharides were extracted from raspberry fruits u-sing a multi-enzyme method and further purified by DEAE-52 cellulose column chromatography to obtain the purified fraction ERP-1.The molecular weight,degree of polymerization(DP),functional groups,monosaccharide composition,and glycosidic linkages of ERP-1 were characterized by high-performance gel permeation chromatography(HPGPC),ultra performance liquid chromatography-tandem mass(UP-LC-MS/MS),fourier transform infrared spectroscopy(FT-IR),ion chromatography(IC),and gas chromatography-mass spectrometry(GC-MS).In vitro immunomodulatory activity was evaluated using murine macrophage RAW264.7 cells.The results showed that ERP-1 had a molecular weight of 569 Da and a Dp ranging from 3 to 7.It was composed of glucose(Glc),fructose(Fru),and mannose(Man)at a molar ratio of 0.582:0.371:0.048.Five types of glycosidic linkages were identified,among which →4)-Glcp-(1→ was the most abundant.Notably,ERP-1 significantly reduced the secretion and mRNA expression of nitric oxide(NO),tumor necrosis factor-α (TNF-α),interleukin-6(IL-6),and interleukin-1 β(IL-1 β)in lipopolysaccharide(LPS)-stimulated RAW264.7 cells.Conversely,it also enhanced the production and mRNA expression of these cytokines in non-LPS-stimulated RAW264.7 cells.These findings could indi-cate that ERP-1 is an oligosaccharide with immunomodulatory activity in vitro and holds promising potential for applications in functional foods and pharmaceutical products.关键词
树莓/低聚糖/结构表征/体外免疫调节活性调节Key words
raspberry(Rubus idaeus L.)/oligosaccharides/structural characterization/in vitro bidirectional immune activity regulation引用本文复制引用
车广学,杨永晶,韩乐,姬一鸣..树莓低聚糖ERP-1结构表征及体外免疫调节活性研究[J].食品与发酵工业,2026,52(15):63-71,9.基金项目
青海省"昆仑英才高端创新创业人才"计划培养人才项目 （）
青海大学生态环境工程学院生态学世界一流学科研究生科技创新项目(2024-STXY-Y28) （2024-STXY-Y28）
