食品科学2026，Vol.47Issue(17)：109-119,11.DOI:10.7506/spkx1002-6630-20260302-007
转录因子PbWRKY29在梨果防御扩展青霉侵染中的作用
Role of Transcription Factor PBWRKY29 in Pear Fruit Defense against Penicillium expansum Infection
摘要
Abstract
Pear fruit are often infected with Penicillium expansum during storage.Therefore,analyzing the regulatory mechanisms of relevant transcription factors on the defense of pear fruit against P.expansum are of great significance.Our previous research found that the transcription factor PbWRKY29 may play an important role in disease resistance in pear fruit.However,the underlying mechanism remains unclear.In this study,we conducted a bioinformatics-based analysis of the physicochemical properties of the PbWRKY29 protein and experimentally investigated its functional role and mechanism of action in pear fruit defense against P.expansum.Our results showed that the protein was composed of 20 amino acids and was characterized as an acidic,unstable,and highly hydrophilic non-transmembrane protein lacking a signal peptide,primarily localized to the nucleus.Its secondary structure comprises α-helices,extended strands,and random coils.Transient overexpression of PbWRKY29 in pear fruit enhanced the resistance to P.expansum.PbWRKY29 reinforces the resistance of pear fruit against P.expansum by positively regulating the expression of ethylene biosynthesis-related genes(PbACS1,PbACS7,PbACO1,PbACO2,and PbSAMB2)and defense-related genes(PRH,PRB1,and PRMS),thereby promoting the accumulation of H2O2,suppressing the production rate of superoxide anion radical,and enhancing the activities of antioxidant enzymes(CAT,SOD,POD,and PPO).These findings provide a new theoretical basis for an in-depth understanding of the molecular mechanisms underlying postharvest defense against P.expansum infection in pear fruit.关键词
梨果/扩展青霉/生物防治/PbWRKY29基因Key words
pear fruit/Penicillium expansum/biocontrol/PbWRKY29分类
轻工纺织引用本文复制引用
赵利娜,任晓玥,李小娟,张晓云,杨其亚,王凯利,张红印..转录因子PbWRKY29在梨果防御扩展青霉侵染中的作用[J].食品科学,2026,47(17):109-119,11.基金项目
国家自然科学基金面上项目(32372401 （）
32372778 （）
32472414 （）
32472804) （）
江苏高校青蓝工程项目 （）
