食品工业科技2026，Vol.47Issue(19)：43-52,10.DOI:10.13386/j.issn1002-0306.2025120027
基于复合QuEChERS净化和UPLC-MS/MS的禽蛋多类禁限用兽药残留筛查与定量分析
Screening and Quantitative Analysis of Multiple Classes of Prohibited/Restricted Veterinary Drug Residues in Poultry Eggs Based on Composite QuEChERS Purification and UPLC-MS/MS
摘要
Abstract
To address the growing need for multi-class,high-throughput monitoring of veterinary drug residues in poultry egg products and the deficiencies of existing methods in detection scope,sensitivity and matrix applicability,a comprehen-sive method for the simultaneous determination of 53 banned and restricted veterinary drugs from 9 classes in chicken,duck,goose,and pigeon eggs was developed.The sample preparation procedure was systematically optimized,including the extraction solvent and QuEChERS purification sorbents.The optimized method involved extraction with 0.2 mol/L Na2EDTA-Mcllvaine buffer solution and acetonitrile,followed by cleanup using a composite of C18,NH2,and PSA sor-bents.The target analytes were then determined by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The results showed that all 53 veterinary drugs exhibited good linearity(R2≥0.9903).The limits of detec-tion(LOD)and the limits of quantification(LOQ)ranged from 1 μg/kg to 4 μg/kg and 2 μg/kg to 10 μg/kg,respectively,which all met or exceeded the maximum residue limits specified in the GB 31650.1-2022 National food safety standard-Maximum residue limits for 41 veterinary drugs in foods.Average recoveries at three spiked levels ranged from 61.2％to 113.6％,with relative standard deviations(RSDs)between 0.1％and 18.9％.The developed method was successfully applied to screen 53 batches of actual poultry egg samples,and eight batches were found to contain target compounds,and the results were consistent with those obtained by the national standard methods.The proposed method successfully solves the problem of simultaneous detection caused by large differences in the physical and chemical properties of drugs and complex poultry egg matrices,and has the advantages of simple sample pretreatment,fast analysis speed(10 min),high sensitivity,accuracy and reliability.It is suitable for high-throughput screening and precise supervision of multi-class vet-erinary drug residues in various poultry egg matrices.关键词
复合QuEChERS净化/超高效液相色谱-串联质谱/禁限用兽药/禽蛋Key words
composite QuEChERS purification/ultra-performance liquid chromatography-tandem mass spectrometry/banned and restricted veterinary drugs/poultry eggs分类
轻工纺织引用本文复制引用
张静,马丽娜,刘茵茵,周倩,万玉,高玉时,唐修君..基于复合QuEChERS净化和UPLC-MS/MS的禽蛋多类禁限用兽药残留筛查与定量分析[J].食品工业科技,2026,47(19):43-52,10.基金项目
农产品质量安全风险评估(20254071). （20254071）
