食品工业科技2026，Vol.47Issue(19)：71-78,8.DOI:10.13386/j.issn1002-0306.2025090065
猴头菇多糖的制备、结构表征及其对皮质酮诱导PC12细胞损伤的保护作用
Preparation,Structural Characterization,and Protective Effects of Hericium erinaceus Polysaccharide against Corticosterone-induced Injury in PC12 Cells
摘要
Abstract
This study aimed to clarify the structural characteristics of Hericium erinaceus polysaccharides and their protec-tive effects against corticosterone-induced injury in PC12 cells.HEP was extracted and purified from the fruiting bodies of Hericium erinaceus by water extraction-alcohol precipitation and column chromatography.Its component contents were determined using the phenol-sulfuric acid method,Coomassie brilliant blue method,and other techniques.Structural chara-cterization was performed via Fourier transform infrared spectroscopy,ultraviolet full-wavelength scanning,molecular weight determination,scanning electron microscopy,and Congo red assay.A PC12 cell injury model induced by 200 μg/mL corticosterone was established,and the protective effects of HEP were investigated using the CCK-8 assay,lactate dehydro-genase release detection,and flow cytometry.The results showed that HEP had a total sugar content of 75.63％,containing 8.35％protein and 7.93％uronic acid,with a weight-average molecular weight of 1.94×104 Da.It possessed a β-D-glucan backbone structure,exhibited a filamentous network arrangement at the microscopic level,and contained partial triple-helix conformations.Furthermore,HEP could dose-dependently increase the viability of damaged PC12 cells(up to 81.28％),sig-nificantly reduce LDH release(down to 362.3 U/L),and decrease the cell apoptosis rate(down to 3.77％).In conclusion,HEP exerts a definite protective effect against corticosterone-induced neurocyte injury and can be further studied as a potential functional food component related to antidepressant effects.关键词
猴头菇多糖/结构表征/皮质酮/PC12细胞/抗抑郁Key words
Hericium erinaceus polysaccharides/structural characterization/corticosterone/PC12 cells/antidepressant分类
轻工纺织引用本文复制引用
龚频,王洁玉,王佳婷,龙辉,樊珂如,杨文娟,李楠..猴头菇多糖的制备、结构表征及其对皮质酮诱导PC12细胞损伤的保护作用[J].食品工业科技,2026,47(19):71-78,8.基金项目
陕西省重点产业创新链(群)-农业领域(2024NC-ZDCYL-04-14) （群）
陕西省重点项目-关键核心技术攻关-农业农村领域(2024NC2-GJHX-25) （2024NC2-GJHX-25）
西安市未央区科技计划(202416). （202416）
