食品与发酵工业2026，Vol.52Issue(17)：1-9,中插1-中插4,13.DOI:10.13995/j.cnki.11-1802/ts.045263
基于甘薯渣碳源与补料策略的白色链霉菌生产ε-聚赖氨酸研究
Efficient ε-poly-L-lysine production of Streptomyces albulus using sweet potato residue as carbon source and feeding strategy
摘要
Abstract
ε-Poly-L-lysine(ε-PL),a natural and broad-spectrum preservative,is extensively utilized in food,cosmetic and pharma-ceutical industries.Currently,ε-PL is mainly produced through submerged fermentation by Streptomyces albulus.The carbon source glucose generally accounts for most of the ε-PL production costs.Therefore,developing cheaper and alternative carbon sources is crucial for ε-PL fermentation.In this study,sweet potato residue hydrolysate(SPRH)used as carbon sources for ε-PL production by S.albulus ZY1 was investigated.When the initial glucose concentration in the SPRH was 50 g/L,after 72 h of shake flask fermentation,the ε-PL titer achieved 1.55 g/L.In addition,compared with the M3G medium(a conventional control medium using pure glucose as the sole carbon source),the activities of glucose-6-phosphate dehydrogenase and ε-PL synthetase in the SPRH increased by 14.3％and 6.4％at 48 h,respectively.Subsequently,transcriptome analysis showed that 37 upregulated genes and 76 downregulated genes were identified.Among these,the ffs gene,which is involved in the protein translocation process mediated by the signal recognition particle(SRP),was upregu-lated.Finally,in fed-batch fermentation with a 5 L bioreactor,ε-PL titer reached 39.09 g/L with a productivity of 4.89 g/(L·d)at the end of fermentation.In summary,this study provided a reference for efficient ε-PL production using agricultural residues as economical carbon sources.关键词
ε-聚赖氨酸/甘薯渣/发酵/转录组/白色链霉菌Key words
ε-Poly-L-lysine/sweet potato residue/fermentation/transcriptome analysis/Streptomyces albulus引用本文复制引用
徐畅,周运峰,张迪,王冰雁,宁海怡,白净,李良智,纪丽莲,扶教龙..基于甘薯渣碳源与补料策略的白色链霉菌生产ε-聚赖氨酸研究[J].食品与发酵工业,2026,52(17):1-9,中插1-中插4,13.基金项目
国家自然科学基金面上项目(22478268) （22478268）
江苏省大学生创新创业训练计划项目(23200120217) （23200120217）
